Chromatography / Stationary phases
ARION - CHROMSERVIS
| Packing Material |
Particle Size (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
| Plus C18 |
1.7, 2.2, 3, 5, 10, 15 |
100 |
420 |
18 |
1.5-10 |
| Polar C18 |
2.2, 3, 5, 10, 15 |
120 |
325 |
16 |
1.5-7.0 |
| C8 |
3, 5 |
120 |
325 |
11 |
2.0-7.0 |
| Phenyl-butyl |
2.2, 3, 5 |
100 |
300 |
12 |
1.5-7.5 |
| NH2 |
2.2, 3, 5 |
120 |
325 |
5 |
2.0-6.5 |
| CN |
3, 5, 10 |
120 |
325 |
8 |
2.0-7.0 |
| HILIC Plus |
2.2, 3, 5 |
120 |
420 |
- |
1.5-7.0 |
| Si |
2.2, 3, 5, 10 |
100 |
420 |
- |
1.5-7.0 |
More information is available at www.arionchromatography.com. You will find Column care guide there.
CHROMSHELL - CHROMSERVIS
KINETEX - PHENOMENEX
| Packing Material |
Particle Size (µm) |
Pore Size (Å) |
Effective Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
| Kinetex XB-C18 |
5, 2.6 |
100 |
200 |
10 |
1.5-8.5* |
| Kinetex C18 |
5, 2.6 |
100 |
200 |
12 |
1.5-8.5* |
| Kinetex C8 |
2.6 |
100 |
200 |
8 |
1.5-8.5* |
| Kinetex PFP |
5, 2.6 |
100 |
200 |
9 |
1.5-8.5* |
| Kinetex HILIC |
2.6 |
100 |
200 |
0 |
2.0-7.5 |
| Kinetex Phenyl-Hexyl |
5, 2.6 |
100 |
200 |
11 |
1.5-8.5* |
* Columns are pH stable from 1.5 to 10 under isocratic conditions. Columns are pH stable from 1.5 to 8.5 under gradient conditions.
Kinetex 2.6µm columns with ID 2.1mm are pressure stable up to 1000 bar, otherwise up to 600 bar.
Kinetec chore-shell colums can be replace by new ChromShell colums. Just try it.
LUNA - PHENOMENEX
| Packing Material |
Particle Size (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
USP Packing |
| Luna Phenyl-Hexyl |
3,5,10,15 |
100 |
400 |
17.5 |
1.5-10.0 |
L11 |
| Luna Silica (2) |
3,5,10,15 |
100 |
400 |
- |
- |
L3 |
| Luna C5 |
5,10 |
100 |
440 |
12.5 |
1.5-10.0 |
- |
| Luna C8 |
5,10 |
100 |
440 |
14.75 |
1.5-10.0 |
L7 |
| Luna C8 (2) |
3,5,10,15 |
100 |
400 |
13.5 |
1.5-10.0 |
L7 |
| Luna C18 |
5,10 |
100 |
440 |
19 |
1.5-10.0 |
L1 |
| Luna C18 (2) |
2.5,3,5,10,15 |
100 |
400 |
17.5 |
1.5-10.0 |
L1 |
| Luna CN |
3,5,10 |
100 |
400 |
7.0 |
1.5-10.0 |
L10 |
| Luna NH2 |
3,5,10 |
100 |
400 |
9.5 |
1.5-11.0 |
L8 |
| Luna SCX |
5,10 |
100 |
400 |
0.55% Sulfur Load |
2.0-7.0 |
L9 |
| Luna HILIC |
3,5 |
200 |
200 |
- |
1.5-8.0 |
- |
| Luna PFP(2) |
3 5 |
100 |
400 |
5.7 |
1.5-8.0 |
L43 |
GEMINI - PHENOMENEX
| Packing Material |
Particle Size (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
USP Packing |
| Gemini C18 |
3,5,10 |
110 |
375 |
14 |
1.0-12.0 |
L1 |
| Gemini C6-Phenyl |
3,5 |
110 |
375 |
12 |
1.0-12.0 |
L11 |
| Gemini NX |
3,5,10 |
110 |
375 |
14 |
1.0-12.0 |
L1 |
SYNERGI - PHENOMENEX
ONYX - PHENOMENEX
| Packing Material |
Macropore Size (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
USP Packing |
| Onyx Silica |
2 |
130 |
300 |
0 |
2.0-7.5 |
- |
| Onyx C8 |
2 |
130 |
300 |
11 |
2.0-7.5 |
- |
| Onyx C18 |
2 |
130 |
300 |
18 |
2.0-7.5 |
- |
JUPITER - PHENOMENEX
| Packing Material |
Particle Size (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
USP Packing |
| Jupiter C4 |
5,10,15 |
300 |
170 |
5.0 |
1.5-10.0 |
L26 |
| Jupiter C5 |
5,10,15 |
300 |
170 |
5.5 |
1.5-10.0 |
- |
| Jupiter C18 |
5,10,15 |
300 |
170 |
13.3 |
1.5-10.0 |
L1 |
| Jupiter Proteo C12 |
4,10 |
90 |
475 |
15.0 |
1.5-10.0 |
- |
GraceSmart - GRACE
| Packing Material |
Particle Size (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
USP Packing |
| GraceSmart C18 |
3,5 |
120 |
220 |
10 |
2.0-9.0 |
L1 |
Alltech® Prevail - GRACE
Nano / Capillary LC Column ProteCol - SGE
| Packing Material |
Particle Size (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Range |
USP Packing |
| ProteCol C18 |
3 |
120/300 |
350 |
17 |
2.0-7.5 |
L1 |
| ProteCol C8 |
3 |
120/300 |
350 |
10 |
2.0-7.5 |
L7 |
| ProteCol C4 |
3 |
120/300 |
350 |
|
2.0-7.5 |
L26 |
| ProteCol SCX |
3 |
120/300 |
350 |
|
2.0-7.5 |
L9 |
Dosing pumps
Dosing pumps are used in many applications, in laboratories and within industry. Often we see the needs, when we require dose liquids under specific conditions:
- High pressure dosing (reaction chambers, aparatures)
- High temperatures dosing
- Injection of reactive substances
- Dosing of viscous media
For all above mentioned applications it is possible to use technology that has been proven in the area of high performance liquid chromatography (HPLC). These are double-piston pumps AZURA (Knauer), which use sphire pistons enabling precise continuous dosing under high pressure. These pumps can work with flow of 0,01 to 1000 ml/min, under temperatures of -10°C to +120°C and with viscous media up to 1000 mPa.s.
The pumps can be especially equipped so they can be used in specific application like hazardous areas.
Nice example of use of AZURA pumps is dosing of sulphur trioxide in production of methanesulfonic acid (MSA).
Materials
Pump head are available in following materials:
- Ceramic
- Hastelloy C-276
- Stainless steel
- Titanum
- Stainless steel/Titanum
Further information about models are available here.
About Flash column stationary phases
Here you will find more information about stationary phases used in reversed phase Flash chromatography.
Reversed phases
puriFl
ash® RP-AQ
60Å - 500 m2/g
15 & 30 μm
RP-alkyl, 6% Carbon
End-capping: mixed
pH stability: 2.0 to 7.5
Separation/purification of strongly and moderately polar molecules.
puriFlash® C18-AQ
100Å - 300 m2/g
5, 10, 15 & 30 μm
C18 mono-functional, 14% Carbon
End-capping: mixed
pH stability: 2.0 to 7.5
Separation/purification of moderately polar and non-polar molecules.
puriFlash® C18-HP
100Å - 300 m2/g
5, 10, 15, 30 & 50 μm
C18 mono-functional, 16,5% Carbon
End-capping: one-step
pH stability: 1.5 to 7.5
This is an excellent choice for routine reverse phase purifications.
Uptisphere® Strategy™ C18-HQ
100Å - 425 m2/g
1.7, 2.2, 3, 5, 10, 15 μm
C18 mono-functional, 19% Carbon
End-capping: multi-step
pH stability: 1.0 to 10.0
Suitable for many pharmaceutical applications and routine methods.
puriFlash® C18-XS
100Å - 300 m2/g
5, 10, 3, 15 & 30 μm
C18 mono-functional, 17% Carbon
End-capping: multi-step
pH stability: 1.0 to 10.0
It is an excellent phase for the complete separation of basic molecules.
There are mor phases available. Contact us for more information for suitable stationary phase for Flahs/Purification chromatography.
puriFlash® Flash Cartridges
Interchim developed new technique for flash chromatography - Ultra Performance Flash Purification (UPFP) using special flash cartridges. The flash cartridges are in the form of regular or irregular silica. UPFP enables to run purifications with high purity of the yield and less solvent use.
Flash column selection
Flash column celection is available at this page.
more…
Chromatography, either GC, HPLC, SPE, FLASH or preparative, uses many types of stationary phases. Here you wil find detailed information about stationary phases.
Stationary phases for analytical separation
Stationary phases for sample preparation
- SPE
- dSPE (QuEChERS)
- IAC (Immunoaffinity Columns)
- MEPS (Micro Extraction by Packed Sorbent)
- FLASH
- BULK (media for preparative chromatography)
This article is under construction.
Kinetex - PHENOMENEX
*Columns are pH stable from 1.5 to 10 under isocratic conditions. Columns are pH stable from 1.5 to 8.5 under gradient conditions.
All Kinetex 1.3 and 1.7µm columns are pressure stable up to 1000 bar. Kinetex 2.6µm columns with ID 2.1mm are pressure stable up to 1000 bar, otherwise up to 600 bar.
PINNACLE DB - RESTEK
(Application Specific Phase)
- Proprietary phase specially designed for the separation of all types of pesticides.
- When used in parallel with the Zebron MR-1 and MR-2 column, provides baseline resolution and confirmation of all 20.chlorinated pesticides regulated under EPA Method 8081 in <10 min.
- MS Certified phases provide low bleed performance for pesticide confirmation by MS.
- Low activity, decreased breakdown of sensitive pesticides such as DDT.
- Provides robust column performance for high temperature bake outs.
- ESC Equipped.
Zebron MultiResidue columns provide baseline resolution of all EPA Method 8081A chlorinated pesticides in less than 10 minutes. In calibration curves ranging from 5-250 ppb, the columns provided results that far exceeded the EPA requirements for % breakdown of DDT and Endrin, linearity (% RSD), and resolution of critical isomers.
The unique selectivity offered by each phase allows for increased resolution of critical compounds vs. standard 5ms type phases. Retention time data is available for over 300 different pesticides on GC/MS, further simplifying new method development.
Applications
- Aroclors/PCBs
- Multi-pesticide Residue Methods
- Haloacetic Acids
- Nitrogen Containing Pesticides
- Organochlorine Pesticides
- Organophosphorous Pesticides
- Herbicides
- Insecticides
Temperature limits
| ID (mm) |
df(µm) |
Temperature limits (°C) |
| 0.25 |
0.25 |
-60 to 320/340 |
| 0.32 |
0.50 |
-60 to 320/340 |
| 0.53 |
0.50 |
-60 to 320/340 |
(35% Phenyl 65% Dimethylpolysiloxane Phase)
- First non-metal, 35 % phenyl columns stable to 400 ˚C
- Robust column for high temperature analysis
- Great for high molecular weight compounds
- Eliminate carry-over with high temperature bake outs
- Longer lifetime with rugged high temperature, polyimide coated, fused silica tubing
- Temperature Limits: -60 to 400 °C (Isothermal/TPGC)
- Equivalent to USP Phase G42
High temperature stable intermediate polarity GC column used to effectively resolve compounds that have similar boiling points, but different functionalities. High temperature limits also allow you to bake off contaminants and extend column lifetime.
Applications
- Amines
- Pesticides
- Aroclors
- Semivolatiles
- Drugs of Abuse
- Steroids
Temperature limits
| ID (mm) |
df(µm) |
Temperature limits (°C) |
| 0.25 |
0.25 |
-60 to 400 |
| 0.32 |
0.25 |
-60 to 400 |
(Application Specific Phase)
- Proprietary phase specially designed for the separation of all types of pesticides, herbicides, and insecticides.
- When used in parallel with the Zebron MR-1 and MR-2 column, provides baseline resolution and confirmation of all 20 chlorinated pesticides regulated under EPA Method 8081 in <10 min.
- MS Certified phases provide low bleed performance for pesticide confirmation by MS.
- Low activity, decreased breakdown of sensitive pesticides such as DDT.
- Provides robust column performance for high temperature bake outs.
- ESC Equipped.
Zebron MultiResidue columns provide baseline resolution of all EPA Method 8081A chlorinated pesticides in less than 10 minutes. In calibration curves ranging from 5-250 ppb, the columns provided results that far exceeded the EPA requirements for % breakdown of DDT and Endrin, linearity (% RSD), and resolution of critical isomers.
The unique selectivity offered by each phase allows for increased resolution of critical compounds vs. standard 5ms type phases. Retention time data is available for over 300 different pesticides on GC/MS, further simplifying new method development.
Applications
- Aroclors/PCBs
- Multi-pesticide Residue Methods
- Haloacetic Acids
- Nitrogen Containing Pesticides
- Organochlorine Pesticides
- Organophosphorous Pesticides
- Herbicides
- Insecticides
Temperature limits
| ID (mm) |
df(µm) |
Temperature limits (°C) |
| 0.25 |
0.20 |
-60 to 320/340 |
| 0.32 |
0.25 |
-60 to 320/340 |
| 0.53 |
0.50 |
-60 to 320/340 |
