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Chromatography / Stationary phases

HPLC Phases

ARION - CHROMSERVIS

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
Plus C18 1.7, 2.2, 3, 5, 10, 15 100 420 18 1.5-10
Polar C18 2.2, 3, 5, 10, 15 120 325 16 1.5-7.0
C8 3, 5 120 325 11 2.0-7.0
Phenyl-butyl 2.2, 3, 5 100 300 12 1.5-7.5
NH2 2.2, 3, 5 120 325 5 2.0-6.5
CN 3, 5, 10 120 325 8 2.0-7.0
HILIC Plus 2.2, 3, 5 120 420 - 1.5-7.0
Si 2.2, 3, 5, 10 100 420 - 1.5-7.0

More information is available at www.arionchromatography.com. You will find Column care guide there.

CHROMSHELL - CHROMSERVIS

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
CHROMSHELL® C18 Plus 2.6 85 130 9 1.5-7.5
CHROMSHELL® C18-XB 2.6 85 130 8 1.5-8.0
CHROMSHELL® C18-AB 2.6 85 130 6 1.5-8.0
CHROMSHELL® C18 Polar 2.6 85 130 6.5 1.5-7.0
CHROMSHELL® HILIC 2.6 85 130 - 1.5-7.0
CHROMSHELL® Si 2.6 85 130 - 1.5-7.0

KINETEX - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Effective Surface Area (m2/g) Carbon Load (%) pH Range
Kinetex XB-C18 5, 2.6 100 200 10 1.5-8.5*
Kinetex C18 5, 2.6 100 200 12 1.5-8.5*
Kinetex C8 2.6 100 200 8 1.5-8.5*
Kinetex PFP 5, 2.6 100 200 9 1.5-8.5*
Kinetex HILIC 2.6 100 200 0 2.0-7.5
Kinetex Phenyl-Hexyl 5, 2.6 100 200 11 1.5-8.5*

* Columns are pH stable from 1.5 to 10 under isocratic conditions. Columns are pH stable from 1.5 to 8.5 under gradient conditions.

Kinetex 2.6µm columns with ID 2.1mm are pressure stable up to 1000 bar, otherwise up to 600 bar.

Kinetec chore-shell colums can be replace by new ChromShell colums. Just try it.

LUNA - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Luna Phenyl-Hexyl 3,5,10,15 100 400 17.5 1.5-10.0 L11
Luna Silica (2) 3,5,10,15 100 400 - - L3
Luna C5 5,10 100 440 12.5 1.5-10.0 -
Luna C8 5,10 100 440 14.75 1.5-10.0 L7
Luna C8 (2) 3,5,10,15 100 400 13.5 1.5-10.0 L7
Luna C18 5,10 100 440 19 1.5-10.0 L1
Luna C18 (2) 2.5,3,5,10,15 100 400 17.5 1.5-10.0 L1
Luna CN 3,5,10 100 400 7.0 1.5-10.0 L10
Luna NH2 3,5,10 100 400 9.5 1.5-11.0 L8
Luna SCX 5,10 100 400 0.55% Sulfur Load 2.0-7.0 L9
Luna HILIC 3,5 200 200 - 1.5-8.0 -
Luna PFP(2) 3 5 100 400 5.7 1.5-8.0 L43

GEMINI - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Gemini C18 3,5,10 110 375 14 1.0-12.0 L1
Gemini C6-Phenyl 3,5 110 375 12 1.0-12.0 L11
Gemini NX 3,5,10 110 375 14 1.0-12.0 L1

SYNERGI - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Synergi Max-RP 2.5 100 400 17 1.5-10.0 -
Synergi Hydro-RP 2.5 100 400 19 1.5-7.5 L1
Synergi Polar-RP 2.5 100 440 11 1.5-7.0 L11
Synergi Fusion-RP 2.5 100 440 12 1.5-10.0 L1
Synergi Max-RP 4,10 80 475 17 1.5-10.0 -
Synergi Hydro-RP 4,10 80 475 19 1.5-7.5 L1
Synergi Polar-RP 4,10 80 475 11 1.5-7.0 L11
Synergi Fusion-RP 4,10 80 475 12 1.5-10.0 L1

ONYX - PHENOMENEX

Packing Material Macropore Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Onyx Silica 2 130 300 0 2.0-7.5 -
Onyx C8 2 130 300 11 2.0-7.5 -
Onyx C18 2 130 300 18 2.0-7.5 -

JUPITER - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Jupiter C4 5,10,15 300 170 5.0 1.5-10.0 L26
Jupiter C5 5,10,15 300 170 5.5 1.5-10.0 -
Jupiter C18 5,10,15 300 170 13.3 1.5-10.0 L1
Jupiter Proteo C12 4,10 90 475 15.0 1.5-10.0 -

GraceSmart - GRACE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
GraceSmart C18 3,5 120 220 10 2.0-9.0 L1

Alltech® Prevail - GRACE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Prevail C18 3,5 110 350 17   L1
Prevail C18 Select 3,5 110 350 15   L1
Prevail C8 3,5 110 350 8   L7
Prevail Phenyl 3,5 110 350 7   L11
Prevail Cyano (CN) 3,5 110 350 -   L10
Prevail Amino (NH2) 3,5 110 350 -   L8
Prevail Silica 3,5 110 350 -   L3
Prevail Organic Acid 3,5 110 350 -   -
Carbohydrate ES (polymer) 5 - - -   -

Nano / Capillary LC Column ProteCol - SGE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
ProteCol C18 3 120/300 350 17 2.0-7.5 L1
ProteCol C8 3 120/300 350 10 2.0-7.5 L7
ProteCol C4 3 120/300 350   2.0-7.5 L26
ProteCol SCX 3 120/300 350   2.0-7.5 L9

 

 

 

 

 

 


Dosing pumps

Dosing pumps

Dosing pumps are used in many applications, in laboratories and within industry. Often we see the needs, when we require dose liquids under specific conditions:

  • High pressure dosing (reaction chambers, aparatures)
  • High temperatures dosing
  • Injection of reactive substances
  • Dosing of viscous media

Pump HeadFor all above mentioned applications it is possible to use technology that has been proven in the area of high performance liquid chromatography (HPLC). These are double-piston pumps AZURA (Knauer), which use sphire pistons enabling precise continuous dosing under high pressure. These pumps can work with flow of 0,01 to 1000 ml/min, under temperatures of -10°C to +120°C and with viscous media up to 1000 mPa.s.

The pumps can be especially equipped so they can be used in specific application like hazardous areas.

Nice example of use of AZURA pumps is dosing of sulphur trioxide in production of methanesulfonic acid (MSA).

Materials

Pump head are available in following materials:

  • Ceramic
  • Hastelloy C-276
  • Stainless steel
  • Titanum
  • Stainless steel/Titanum

Further information about models are available here.

 


puriFlash RP

About Flash column stationary phases

Here you will find more information about stationary phases used in reversed phase Flash chromatography.

Reversed phases

puriFlRP-AQash® RP-AQ

60Å - 500 m2/g

15 & 30 μm

RP-alkyl, 6% Carbon

End-capping: mixed

pH stability: 2.0 to 7.5

Separation/purification of strongly and moderately polar molecules.

 

 


C18-AQ

puriFlash® C18-AQ

100Å - 300 m2/g

5, 10, 15 & 30 μm

C18 mono-functional, 14% Carbon

End-capping: mixed

pH stability: 2.0 to 7.5

Separation/purification of moderately polar and non-polar molecules.

 

 


C18-HP

puriFlash® C18-HP

100Å - 300 m2/g

5, 10, 15, 30 & 50 μm

C18 mono-functional, 16,5% Carbon

End-capping: one-step

pH stability: 1.5 to 7.5

This is an excellent choice for routine reverse phase purifications.

 

 

 


Uptisphere® Strategy™ C18-HQ

Uptisphere® Strategy™ C18-HQ

100Å - 425 m2/g

1.7, 2.2, 3, 5, 10, 15 μm

C18 mono-functional, 19% Carbon

End-capping: multi-step

pH stability: 1.0 to 10.0

Suitable for many pharmaceutical applications and routine methods.

 

 

 


puriFlash® C18-XS

puriFlash® C18-XS

100Å - 300 m2/g

5, 10, 3, 15 & 30 μm

C18 mono-functional, 17% Carbon

End-capping: multi-step

pH stability: 1.0 to 10.0

It is an excellent phase for the complete separation of basic molecules.

 

 

 


There are mor phases available. Contact us for more information for suitable stationary phase for Flahs/Purification chromatography.

 

 


puriFlash

puriFlash® Flash Cartridges

Interchim developed new technique for flash chromatography - Ultra Performance Flash Purification (UPFP) using special flash cartridges. The flash cartridges are in the form of regular or irregular silica. UPFP enables to run purifications with high purity of the yield and less solvent use. 

puriFlash cartridges

Flash column selection

Flash column celection is available at this page.


Stationary phases

Chromatography phasesChromatography, either GC, HPLC, SPE, FLASH or preparative, uses many types of stationary phases. Here you wil find detailed information about stationary phases.

Stationary phases for analytical separation

Stationary phases for sample preparation

  • SPE
  • dSPE (QuEChERS)
  • IAC (Immunoaffinity Columns)
  • MEPS (Micro Extraction by Packed Sorbent)
  • FLASH
  • BULK (media for preparative chromatography)

 



UHPLC Phases

Kinetex - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Effective Surface Area (m2/g) Carbon Load (%) pH Range
Kinetex C18 1.3 100 200 12 1.5-8.5*
Kinetex C18 1.7 100 200 12 1.5-8.5*
Kinetex C18 2.6 100 200 12 1.5-8.5*
Kinetex XB-C18 1.7 100 200 10 1.5-8.5*
Kinetex XB-C18 2.6 100 200 10 1.5-8.5*
Kinetex C8 1.7 100 200 8 1.5-8.5*
Kinetex C8 2.6 100 200 8 1.5-8.5*
Kinetex PFP 1.7 100 200 9 1.5-8.5*
Kinetex PFP 2.6 100 200 9 1.5-8.5*
Kinetex HILIC 1.7 100 200 0 2.0-7.5
Kinetex HILIC 2.6 100 200 0 2.0-7.5
Kinetex Phenyl-Hexyl 1.7 100 200 11 1.5-8.5*
Kinetex Phenyl-Hexyl 2.6 100 200 11 1.5-8.5*

*Columns are pH stable from 1.5 to 10 under isocratic conditions. Columns are pH stable from 1.5 to 8.5 under gradient conditions.

All Kinetex 1.3 and 1.7µm columns are pressure stable up to 1000 bar. Kinetex 2.6µm columns with ID 2.1mm are pressure stable up to 1000 bar, otherwise up to 600 bar.

PINNACLE DB - RESTEK

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
Pinnacle DB C18 1.9 140   11 2.5-10.0
Pinnacle DB Aqueous C18 1.9 140   6 2.5-7.5
Pinnacle DB C8 1.9 140   6 2.5-10.0
Pinnacle DB CN 1.9 140   4 2.5-7.5
Pinnacle DB PFP 1.9 140   6 2.5-7.5
Pinnacle DB Biphenyl 1.9 140   8 2.5-7.5
Pinnacle DB IBD 1.9 140     2.5-7.5
Pinnacle DB Silica 1.9 140   - 2.5-10.0

ZB-MultiResidue 1

(Application Specific Phase)GC columns

  • Proprietary phase specially designed for the separation of all types of pesticides.
  • When used in parallel with the Zebron MR-1 and MR-2 column, provides baseline resolution and confirmation of all 20.chlorinated pesticides regulated under EPA Method 8081 in <10 min.
  • MS Certified phases provide low bleed performance for pesticide confirmation by MS.
  • Low activity, decreased breakdown of sensitive pesticides such as DDT.
  • Provides robust column performance for high temperature bake outs.
  • ESC Equipped.

Zebron MultiResidue columns provide baseline resolution of all EPA Method 8081A chlorinated pesticides in less than 10 minutes. In calibration curves ranging from 5-250 ppb, the columns provided results that far exceeded the EPA requirements for % breakdown of DDT and Endrin, linearity (% RSD), and resolution of critical isomers.

The unique selectivity offered by each phase allows for increased resolution of critical compounds vs. standard 5ms type phases. Retention time data is available for over 300 different pesticides on GC/MS, further simplifying new method development.

Applications
  • Aroclors/PCBs
  • Multi-pesticide Residue Methods
  • Haloacetic Acids
  • Nitrogen Containing Pesticides
  • Organochlorine Pesticides
  • Organophosphorous Pesticides
  • Herbicides
  • Insecticides
Temperature limits
ID (mm) df(µm) Temperature limits (°C)
0.25 0.25 -60 to 320/340
0.32 0.50 -60 to 320/340
0.53 0.50 -60 to 320/340

ZB-35 HT Interferno

(35% Phenyl 65% Dimethylpolysiloxane Phase)GC columns

  • First non-metal, 35 % phenyl columns stable to 400 ˚C
  • Robust column for high temperature analysis
  • Great for high molecular weight compounds
  • Eliminate carry-over with high temperature bake outs
  • Longer lifetime with rugged high temperature, polyimide coated, fused silica tubing
  • Temperature Limits: -60 to 400 °C (Isothermal/TPGC)
  • Equivalent to USP Phase G42

 

High temperature stable intermediate polarity GC column used to effectively resolve compounds that have similar boiling points, but different functionalities. High temperature limits also allow you to bake off contaminants and extend column lifetime.

Applications
  • Amines
  • Pesticides
  • Aroclors
  • Semivolatiles
  • Drugs of Abuse
  • Steroids
Temperature limits
ID (mm) df(µm) Temperature limits (°C)
0.25 0.25 -60 to 400
0.32 0.25 -60 to 400

ZB-MultiResidue 2

(Application Specific Phase)GC columns

  • Proprietary phase specially designed for the separation of all types of pesticides, herbicides, and insecticides.
  • When used in parallel with the Zebron MR-1 and MR-2 column, provides baseline resolution and confirmation of all 20 chlorinated pesticides regulated under EPA Method 8081 in <10 min.
  • MS Certified phases provide low bleed performance for pesticide confirmation by MS.
  • Low activity, decreased breakdown of sensitive pesticides such as DDT.
  • Provides robust column performance for high temperature bake outs.
  • ESC Equipped.

Zebron MultiResidue columns provide baseline resolution of all EPA Method 8081A chlorinated pesticides in less than 10 minutes. In calibration curves ranging from 5-250 ppb, the columns provided results that far exceeded the EPA requirements for % breakdown of DDT and Endrin, linearity (% RSD), and resolution of critical isomers.

The unique selectivity offered by each phase allows for increased resolution of critical compounds vs. standard 5ms type phases. Retention time data is available for over 300 different pesticides on GC/MS, further simplifying new method development.

Applications
  • Aroclors/PCBs
  • Multi-pesticide Residue Methods
  • Haloacetic Acids
  • Nitrogen Containing Pesticides
  • Organochlorine Pesticides
  • Organophosphorous Pesticides
  • Herbicides
  • Insecticides
Temperature limits
ID (mm) df(µm) Temperature limits (°C)
0.25 0.20 -60 to 320/340
0.32 0.25 -60 to 320/340
0.53 0.50 -60 to 320/340

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