ENEN CZCZ SKSK PLPL RURU

Chromatography / Stationary phases

SPE Phases

SPE kolonky

 

This site encloses SPE phases overview includint their technical parameters. Further information about the products are available in the product catalogue.

 

 

 

 

 

Clean up SPE columns 

Reverse phase hydrophobic 

Phase Pore volume  (cm3/g) Pore Size (A) Surface Area (m2/g) Carbon Load (%) End Capping Feature
C2 Ethyl 0.77 60  500 6.6 YES/NO  
C4 n-Butyl  0.77 60 500 8.5 YES  
C8 Octyl 0.77 60 500 11.1 YES/NO  
C18 Octadecyl 0.77 60 500 21.7 YES/NO  
C30 Tricontyl 0.77 60 500 20.0 YES  
Cyclohexyl 0.77 60 500 11.6 YES/NO  
Phenyl 0.77 60 500 11.0 YES/NO  

Normal phase hydrophilic 

Phase Pore volume  (cm3/g) Pore Size (A) Surface Area (m2/g) Carbon Load (%)   Feature
Silica 0.77 60  500 N/A    
Diol 0.99 60 500 8.0    
Cyanopropyl 0.77 60 500 9.0    
Florisil 0.82 60 500 N/A    
Alumina, Acidic   60 500 N/A    
Alumina, Basic   60 500 N/A    
Alumina, Neutral   60 500 N/A    
Carbon       N/A   120/140 mesh

Ion Exchange - Anion Exchange

Phase Pore volume  (cm3/g) pKa Pore Size (A) Surface Area (m2/g) Carbon Load (%) Exchange (meq/g)
Aminopropyl (1 amine) 0.77 9.8 60 500 6.65 0.31
N-2 Aminoethyl (1/2 amine) 0.77 10.1; 10.9 60 500 11.1 0.32
Diethylamino (3 amine) 0.77 10.6 60 500 10.6 0.28
Quarternary Amine Chloride 0.77 Always charged 60 500 8.4 0.25
Quarternary Amine Hydroxide 0.77 Always charged 60 500 8.4 0.25
Quarternary Amine Acetate 0.77 Always charged 60 500 8.4 0.25
Quarternary Amine Formate 0.77 Always charged 60 500 8.4 0.25
Polyimine 0.77 Always charged     13.5 0.25

Ion Exchange - Cation Exchange

Phase Pore volume  (cm3/g) pKa Pore Size (A) Surface Area (m2/g) Carbon Load (%) Exchange (meq/g)
Carboxylic Acid 0.77 4.8 60 500 9.2 0.17
Propylsulfonic Acid 0.77 1 60 500 7.1 0.18
Benzenesulfonic Acid 0.77 Always charged 60 500 11.0 0.32
Benzenesulfonic Acid, High Load 0.77 Always charged 60 500 15.0 0.65
Triacetic Acid 0.77   60 500 7.61 Anion 0.17/Cation 0.06

Copolymeric phases

Phase Pore volume  (cm3/g) pKa Pore Size (A) Surface Area (m2/g) Carbon Load (%) Exchange (meq/g)
Aminopropyl + C8 0.77 9,8 60 500 12,3 0,163
Quarternary Amine + C8 0.77 Always charged 60 500 13,6 0,160
Carboxylic Acid + C8 0.77 4,8 60 500 2,5 0,105
Propylsulfonic Acid + C8 0.77 1 60 500 14,62 0,114
Benzenesulfonic Acid + C8 0,77 Always charged 60 500 12.3 0,072
Cyanopropyl + C8 0,77 N/A 60 500 14,6 0,163
Cyclohexyl + C8 0.77 N/A 60 500 N/A N/A

GC Phases

Capillary column

Stationary phases LION

On this page we provide an overview of the supplied stationary phases for gas chromatography (GC). Each is given details of its properties and the applications that are suitable for them. In the product catalog you can then choose a suitable quartz or metal capillary column for GC.

Fused silica capillary columns

 

Stationary phase Temperature range Composition USP Phase
LN-1 -60 to 370°C 100% dimethyl polysiloxane G2
LN-1 MS -60 to 370°C 100% dimethyl polysiloxane G2
LN-1 HT -60 to 430°C 100% dimethyl polysiloxane -
LN-5 -60 to 370°C 5% diphenyl/95% dimethyl polysiloxane G27
LN-5 Sil MS -60 to 370°C 5% diphenyl/95% dimethyl polysiloxane G27
LN-5 MS -60 to 350°C 5% phenyl - arylene - 95% dimethyl polysiloxane G27
LN-5 HT -60 to 430°C 5% diphenyl/95% dimethyl polysiloxane -
LN-35 50 to 360°C 35% diphenyl/65% dimethyl polysiloxane G42
LN-35 HT -60 to 400°C 35% diphenyl/65% dimethyl polysiloxane G42
LN-17 40 to 340°C 50% diphenyl/50% dimethyl polysiloxane G3
LN-624 -20 to 260°C 6% cyanopropylphenyl/94% dimethyl polysiloxane G43
LN-FFAP 40 to 260°C Nitroterephthalic Acid Modified Polyethylene Glycol G35
LN-1701 -20 to 300°C 14% cyanopropylphenyl/86% dimethyl polysiloxane G46
LN-XLB 30 to 360°C Low polarity phases -
LN-XLB-HT 30 až 400°C Low polarity phases  
LN-WAX 40 to 260°C Polyethylene Glycol G16
LN-WAX Plus 20 to 260°C Polyethylene Glycol G16

Phenomenex stationary phases

Fused silica capillary columns
Stationary phase Temperature range Composition USP Phase
ZB-1 -60 to 370°C 100% dimethyl polysiloxane G2
ZB-1 MS -60 to 370°C 100% dimethyl polysiloxane G2
ZB-1 HT Inferno -60 to 430°C 100% dimethyl polysiloxane -
ZB-5 -60 to 370°C 5% diphenyl/95% dimethyl polysiloxane G27
ZB-5 MSi -60 to 370°C 5% diphenyl/95% dimethyl polysiloxane G27
ZB-5 MS -60 to 350°C 5% phenyl - arylene - 95% dimethyl polysiloxane G27
ZB-5 HT Inferno -60 to 430°C 5% diphenyl/95% dimethyl polysiloxane -
ZB-35 50 to 360°C 35% diphenyl/65% dimethyl polysiloxane G42
ZB-35 HT Inferno -60 to 400°C 35% diphenyl/65% dimethyl polysiloxane G42
ZB-50 40 to 340°C 50% diphenyl/50% dimethyl polysiloxane G3
ZB-624 -20 to 260°C 6% cyanopropylphenyl/94% dimethyl polysiloxane G43
ZB-FFAP 40 to 260°C Nitroterephthalic Acid Modified Polyethylene Glycol G35
ZB-1701 -20 to300°C 14% cyanopropylphenyl/86% dimethyl polysiloxane G46
ZB-1701P -20 to300°C 14% cyanopropylphenyl/86% dimethyl polysiloxane G46
ZB-XLB 30 to 360°C Proprietary -
ZB-XLB-HT Inferno 30 to 400°C Proprietary -
ZB-WAX 40 to 260°C Polyethylene Glycol G16
ZB-WAX Plus 20 to 260°C Polyethylene Glycol G16
ZB-MR-1 -60 to 340°C Application-specific phase -
ZB-MR-2 -60 to 340°C Application-specific phase -
ZB-BAC-1&2   Application-specific phase -
ZB-Drug-1   Application-specific phase -
ZB-Bioethanol -60 to 360°C Application-specific phase -
Metal capillary columns
Stationary phase Temperature range Composition USP Phase
ZB-1XT SimDist -60 to 450°C Application-specific phase -

 


UHPLC Phases

Raptor - RESTEK

Packing Material Particle Size (µm) Pore Size (Å) Effective Surface Area (m2/g) Carbon Load (%) pH Range
Raptor ARC-C18 1.8 90 125 proprietary 1.0-8.0
Raptor ARC-C18 2.7 90 130 proprietary 1.0-8.0
Raptor ARC-C18 5.0 90 100 proprietary 1.0-8.0
Raptor C18 1.8 90 125 proprietary 2.0-8.0
Raptor C18 2.7 90 130 proprietary 2.0-8.0
Raptor C18 5.0 90 100 proprietary 2.0-8.0
Raptor Biphenyl 1.8 90 125 proprietary 1.5-8.0
Raptor Biphenyl 2.7 90 130 proprietary 1.5-8.0
Raptor Biphenyl 5.0 90 100 proprietary 1.5-8.0
Raptor Fluorophenyl 1.8 90 125 proprietary 2.0-8.0
Raptor Fluorophenyl 2.7 90 130 proprietary 2.0-8.0
Raptor Fluorophenyl 5.0 90 100 proprietary 2.0-8.0
Raptor HILIC-Si 2.7 90 150 n/a 2.0-8.0
Raptor EtG/EtS 2.7 90 130 proprietary 2.0-8.0

Raptor maximum pressure: 1,034 bar (1.8 μm), 600 bar (2.7 μm); 400 bar (5 μm). For maximum lifetime recommended maximum pressure for 1.8 µm particles is 830 bar.

PINNACLE DB - RESTEK

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
Pinnacle DB C18 1.9 140 150 11 2.5-8.0
Pinnacle DB Aqueous C18 1.9 140 150 6 2.5-8.0
Pinnacle DB C8 1.9 140 150 6 2.5-8.0
Pinnacle DB CN 1.9 140 150 4 2.5-8.0
Pinnacle DB PFP 1.9 140 150 6 2.5-8.0
Pinnacle DB Biphenyl 1.9 140 150 8 2.5-8.0
Pinnacle DB IBD 1.9 140 150 proprietary 2.5-8.0
Pinnacle DB Silica 1.9 140 150 n/a 2.5-8.0

puriFlash

puriFlash® Flash Cartridges

Interchim developed new technique for flash chromatography - Ultra Performance Flash Purification (UPFP) using special flash cartridges. The flash cartridges are in the form of regular or irregular silica. UPFP enables to run purifications with high purity of the yield and less solvent use. 

puriFlash cartridges

Flash column selection

Flash column celection is available at this page.


dSPE (QuEChERS)

Resprep™ QuEChERS Products

QuEChERS Tubes For Extraction and Clean-Up of Pesticide Residues From Food Products

  • Fast, simple sample extraction and cleanup using dSPE.
  • Fourfold increases in sample throughput.
  • Fourfold decreases in material cost.
  • Convenient, ready to use centrifuge tubes with ultra pure, preweighed adsorbent mixes.

 

QuechersQuick, Easy, Cheap, Effective, Rugged, and Safe, the QuEChERS ("catchers") method is based on work done and published by the US Department of Agriculture Eastern Regional Research Center in Wyndmoor, PA.(1) Researchers there were looking for a simple, effective, and inexpensive way to extract and clean pesticide residues from the many varied sample matrices with which they routinely worked. They had been using the Modified Luke Extraction Method, which is highly effective and rugged, but is both labor and glassware intensive, leading to a relatively high cost per sample. Solid phase extraction also had been effective, but the complex matrices the investigators were dealing with required multiple individual cartridges and packings to remove the many classes of interferences, adding costs and complexity to the process. A new method would have to remove sugars, lipids, organic acids, sterols, proteins, pigments and excess water, any of which often are present, but still be easy to use and inexpensive.

 

The researchers developed a simple two-step procedure. First, the homogenized samples are extracted and partitioned, using an organic solvent and salt solution. Then, the supernatant is further extracted and cleaned, using a dispersive SPE technique. Multiple adsorbents are placed in a centrifuge tube, along with the 1mL of organic solvent and the extracted residues partitioned from step 1. The contents are thoroughly mixed, then centrifuged, producing a clean extract ready for a variety of GC or HPLC analytical techniques.(2) Validation and proficiency data for the QuEChERS method are available for a wide variety of pesticides in several common food matrices at www.quechers.com.

 

Using the dispersive SPE approach, the quantity and type of adsorbents, as well as the pH and polarity of the solvent, can be easily adjusted for differing matrix interferences and "difficult" analytes. Results from this approach have been verified and modified at several USDA and Food and Drug Administration labs, and the method now is widely accepted for many types of pesticide residue samples.

 

Restek products make this approach even simpler. The centrifuge tube format, available in 2mL and 15mL sizes, contains magnesium sulfate (to partition water from organic solvent) and PSA* adsorbent (to remove sugars and fatty acids), with or without graphitized carbon (to remove pigments and sterols) or C18 packing (to remove nonpolar interferences). Custom products are available by quote request. If you are frustrated by the time and cost involved with your current approach to pesticide sample cleanup, we suggest you try this simple and economical new method.

We have products compliant with AOAC, Multi-miniresidue and Draft European methods.

Inforamtion about products is available here.

References:

  1. Anastassiades, M., S.J. Lehotay, D. Stajnbaher, F.J. Schenck, Fast and Easy Multiresidue Method Employing Acetonitrile Extraction/Partitioning and "Dispersive Solid-Phase Extraction" for the Determination of Pesticide Residues in Produce, J AOAC International, 2003, vol 86 no 22, pp 412-431.
  2. Schenck, F.J., SPE Cleanup and the Analysis of PPB Levels of Pesticides in Fruits and Vegetables. Florida Pesticide Residue Workshop, 2002

MEPS Phases

MEPS phases

 

SGE

Phase Particle size (µm) Pore Size (A)
Silica 45 60
C2 45 60
C8 45 60
C8+SCX* 45 60
C18 45 60

*C8+SCX BINS are labelled as M1.

The BINs can be used up to 40 - 100 extractions. General preparation time is 1 - 2 minutes. More information about MEPS products are available in the product catalogue.


Stationary phases

Chromatography phasesChromatography, either GC, HPLC, SPE, FLASH or preparative, uses many types of stationary phases. Here you wil find detailed information about stationary phases.

Stationary phases for analytical separation

Stationary phases for sample preparation

  • SPE
  • dSPE (QuEChERS)
  • IAC (Immunoaffinity Columns)
  • MEPS (Micro Extraction by Packed Sorbent)
  • FLASH
  • BULK (media for preparative chromatography)

 


Leak Free SilTite metal ferrules for GC & GC/MS

SilTite ferrules are a unique metal ferrule specifically designed for connecting fused silica GC columns and tubing to mass spectrometer interfaces and injectors. Once fitted, SilTite ferrules provide a continuous leak free connection without the need to re-tighten the nut after a few temperature cycles. SilTite ferrules make Graphite/Vespel® ferrules obsolete for use in GC-MS connections. Their performance and cost effectiveness also makes them ideal for connecting GC columns to injectors and atmospheric detectors.

Why choose SilTite ferrules?

  • Eliminates leaks (See figures below)
  • Never needs re-tightening, even after temperature cycling
  • Ferrule remains permanently fixed to the column but does not adhere to the SilTite nut
  • No contamination from Vespel or graphite materials - 100% metal
  • Ideal for high pressure applications
  • Also available for injector interfaces
  • >500°C maximum temperature

V/G ferrules after 5 cycles

Figure 1. MS trace using a graphite Vespel ferrule after 5 temperature cycles.

SilTite ferrules after 5 cycles

Figure 2. MS trace using a SilTite ferrule after 5 temperature cycles. (Using an MS, no leaks can be detected, even after 400 temperature cycles between 70ºC and 400ºC).


Agilent S/SL inlet seal improvment

Dual Vespel Ring SealWasherless, Leak-Tight Seal for Agilent GCs ensures better tightness and easier handling than original part.

  • Prevents oxygen from permeating the carrier gas, increasing column lifetime.
  • Vespel® ring in top surface reduces operator variability by requiring minimal torque to seal.
  • Vespel® ring in bottom surface simplifies installation—eliminates the washer.

In Agilent split/splitless injection ports, it can be difficult to make and maintain a good seal with a conventional metal inlet disk. The metal-to-metal seal dictates that you apply considerable torque to the reducing nut, and, based on our testing, this does not ensure a leak-tight seal. Over the course of oven temperature cycling, metal seals are prone to leaks, which ultimately can degrade the capillary column and cause other analytical difficulties.

Agilent and Restek seal tightness comparison

Tightness

Patented Dual Vespel® Ring Inlet Seal (Restek) greatly improves injection port performance—it stays sealed, even after repeated temperature cycles, without retightening the reducing nut| This seal features two soft Vespel® rings, one embedded in its top surface and the other embedded in its bottom surface. These rings eliminate the need for a washer, and ensure very little torque is needed to make a leak-tight seal. The rings will not harm the critical seal in the injector body, or any other surface, and are outside the sample flow path. Tests using a high sensitivity helium leak detector show Dual Vespel® Ring Inlet Seals will seal equally effectively at torques from 5 in. lb. to 60 in. lb.

Why trust a metal-to-metal seal when you can make leak-tight seals quickly and easily—and more reliably—without a washer, with a Restek Dual Vespel® Ring Inlet Seal. Use a stainless steel seal for analyses of unreactive compounds. To reduce breakdown and adsorption of active compounds, use a gold-plated or Siltek®-treated seal. The gold surface offers better inertness than untreated stainless steel. Siltek® treatment provides inertness similar to that of a fused silica capillary column.

Seal options

  • Stainless Steel
  • Gold plated
  • Siltek deactivated

 

 


HPLC Phases

ARION - CHROMSERVIS

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
Plus C18 1.7, 2.2, 3, 5, 10, 15 100 420 18 1.5-10
Polar C18 2.2, 3, 5, 10, 15 120 325 16 1.5-7.0
C8 3, 5 120 325 11 2.0-7.0
Phenyl-butyl 2.2, 3, 5 100 300 12 1.5-7.5
NH2 2.2, 3, 5 120 325 5 2.0-6.5
CN 3, 5, 10 120 325 8 2.0-7.0
HILIC Plus 2.2, 3, 5 120 420 - 1.5-7.0
Si 2.2, 3, 5, 10 100 420 - 1.5-7.0

More information is available at www.arionchromatography.com. You will find Column care guide there.

CHROMSHELL - CHROMSERVIS

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
CHROMSHELL® C18 Plus 2.6 85 130 9 1.5-7.5
CHROMSHELL® C18-XB 2.6 85 130 8 1.5-8.0
CHROMSHELL® C18-AB 2.6 85 130 6 1.5-8.0
CHROMSHELL® C18 Polar 2.6 85 130 6.5 1.5-7.0
CHROMSHELL® HILIC 2.6 85 130 - 1.5-7.0
CHROMSHELL® Si 2.6 85 130 - 1.5-7.0

KINETEX - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Effective Surface Area (m2/g) Carbon Load (%) pH Range
Kinetex XB-C18 5, 2.6 100 200 10 1.5-8.5*
Kinetex C18 5, 2.6 100 200 12 1.5-8.5*
Kinetex C8 2.6 100 200 8 1.5-8.5*
Kinetex PFP 5, 2.6 100 200 9 1.5-8.5*
Kinetex HILIC 2.6 100 200 0 2.0-7.5
Kinetex Phenyl-Hexyl 5, 2.6 100 200 11 1.5-8.5*

* Columns are pH stable from 1.5 to 10 under isocratic conditions. Columns are pH stable from 1.5 to 8.5 under gradient conditions.

Kinetex 2.6µm columns with ID 2.1mm are pressure stable up to 1000 bar, otherwise up to 600 bar.

Kinetec chore-shell colums can be replace by new ChromShell colums. Just try it.

LUNA - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Luna Phenyl-Hexyl 3,5,10,15 100 400 17.5 1.5-10.0 L11
Luna Silica (2) 3,5,10,15 100 400 - - L3
Luna C5 5,10 100 440 12.5 1.5-10.0 -
Luna C8 5,10 100 440 14.75 1.5-10.0 L7
Luna C8 (2) 3,5,10,15 100 400 13.5 1.5-10.0 L7
Luna C18 5,10 100 440 19 1.5-10.0 L1
Luna C18 (2) 2.5,3,5,10,15 100 400 17.5 1.5-10.0 L1
Luna CN 3,5,10 100 400 7.0 1.5-10.0 L10
Luna NH2 3,5,10 100 400 9.5 1.5-11.0 L8
Luna SCX 5,10 100 400 0.55% Sulfur Load 2.0-7.0 L9
Luna HILIC 3,5 200 200 - 1.5-8.0 -
Luna PFP(2) 3 5 100 400 5.7 1.5-8.0 L43

GEMINI - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Gemini C18 3,5,10 110 375 14 1.0-12.0 L1
Gemini C6-Phenyl 3,5 110 375 12 1.0-12.0 L11
Gemini NX 3,5,10 110 375 14 1.0-12.0 L1

SYNERGI - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Synergi Max-RP 2.5 100 400 17 1.5-10.0 -
Synergi Hydro-RP 2.5 100 400 19 1.5-7.5 L1
Synergi Polar-RP 2.5 100 440 11 1.5-7.0 L11
Synergi Fusion-RP 2.5 100 440 12 1.5-10.0 L1
Synergi Max-RP 4,10 80 475 17 1.5-10.0 -
Synergi Hydro-RP 4,10 80 475 19 1.5-7.5 L1
Synergi Polar-RP 4,10 80 475 11 1.5-7.0 L11
Synergi Fusion-RP 4,10 80 475 12 1.5-10.0 L1

ONYX - PHENOMENEX

Packing Material Macropore Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Onyx Silica 2 130 300 0 2.0-7.5 -
Onyx C8 2 130 300 11 2.0-7.5 -
Onyx C18 2 130 300 18 2.0-7.5 -

JUPITER - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Jupiter C4 5,10,15 300 170 5.0 1.5-10.0 L26
Jupiter C5 5,10,15 300 170 5.5 1.5-10.0 -
Jupiter C18 5,10,15 300 170 13.3 1.5-10.0 L1
Jupiter Proteo C12 4,10 90 475 15.0 1.5-10.0 -

GraceSmart - GRACE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
GraceSmart C18 3,5 120 220 10 2.0-9.0 L1

Alltech® Prevail - GRACE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Prevail C18 3,5 110 350 17   L1
Prevail C18 Select 3,5 110 350 15   L1
Prevail C8 3,5 110 350 8   L7
Prevail Phenyl 3,5 110 350 7   L11
Prevail Cyano (CN) 3,5 110 350 -   L10
Prevail Amino (NH2) 3,5 110 350 -   L8
Prevail Silica 3,5 110 350 -   L3
Prevail Organic Acid 3,5 110 350 -   -
Carbohydrate ES (polymer) 5 - - -   -

Nano / Capillary LC Column ProteCol - SGE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
ProteCol C18 3 120/300 350 17 2.0-7.5 L1
ProteCol C8 3 120/300 350 10 2.0-7.5 L7
ProteCol C4 3 120/300 350   2.0-7.5 L26
ProteCol SCX 3 120/300 350   2.0-7.5 L9

 

 

 

 

 

 


Copyright © CHROMSERVIS s.r.o., Jakobiho 327, CZ-109 00 Praha 10 ~ Tel: +420 274 021 211 ~ Znalostní databáze ~ e-shop od MyWebdesign.cz