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Preparative LC

External flow cellTask for preparative HPLC systems differs to analytical one. While analytical HPLC task is qualitative and quantitative determination of defined compounds in samples, preparative HPLC task is separation, purification and isolation of value products from mixtures.

Preparative chromatography can be devidet into three main areas:

  • Semi-preparative separations
  • Batch preparative chromatography (pilot or industrial scale)
  • True Counter-current chromatography
  • Simulated moving bed (SMB)
  • Continuous chromatography

Scale definition

Parameter Analytical Semi-preparative Preparative
Column sizes (mm) 120 - 250 x 2 - 4.6 120 - 250 x 8 - 16 120 - 250 x 20 - 62
Particle size (µm) up to 5 5 - 10 higher than 10
Stationary phase (g) up to 5 5 - 30 50 - 450
Tubings 1/16" 1/16" 1/8"
Flow rates (ml/min) 0.1 - 2 5 - 50 100 - 1000
Sample size (mg) 0.01 - 2 0.1 - 50 1 - 700
Flow cell (mm) 10 3 0.5 - 2

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