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Chromatography

HPLC Phases

ARION - CHROMSERVIS

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
Plus C18 1.7, 2.2, 3, 5, 10, 15 100 420 18 1.5-10
Polar C18 2.2, 3, 5, 10, 15 120 325 16 1.5-7.0
C8 3, 5 120 325 11 2.0-7.0
Phenyl-butyl 2.2, 3, 5 100 300 12 1.5-7.5
NH2 2.2, 3, 5 120 325 5 2.0-6.5
CN 3, 5, 10 120 325 8 2.0-7.0
HILIC Plus 2.2, 3, 5 120 420 - 1.5-7.0
Si 2.2, 3, 5, 10 100 420 - 1.5-7.0

More information is available at www.arionchromatography.com. You will find Column care guide there.

CHROMSHELL - CHROMSERVIS

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range
CHROMSHELL® C18 Plus 2.6 85 130 9 1.5-7.5
CHROMSHELL® C18-XB 2.6 85 130 8 1.5-8.0
CHROMSHELL® C18-AB 2.6 85 130 6 1.5-8.0
CHROMSHELL® C18 Polar 2.6 85 130 6.5 1.5-7.0
CHROMSHELL® HILIC 2.6 85 130 - 1.5-7.0
CHROMSHELL® Si 2.6 85 130 - 1.5-7.0

KINETEX - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Effective Surface Area (m2/g) Carbon Load (%) pH Range
Kinetex XB-C18 5, 2.6 100 200 10 1.5-8.5*
Kinetex C18 5, 2.6 100 200 12 1.5-8.5*
Kinetex C8 2.6 100 200 8 1.5-8.5*
Kinetex PFP 5, 2.6 100 200 9 1.5-8.5*
Kinetex HILIC 2.6 100 200 0 2.0-7.5
Kinetex Phenyl-Hexyl 5, 2.6 100 200 11 1.5-8.5*

* Columns are pH stable from 1.5 to 10 under isocratic conditions. Columns are pH stable from 1.5 to 8.5 under gradient conditions.

Kinetex 2.6µm columns with ID 2.1mm are pressure stable up to 1000 bar, otherwise up to 600 bar.

Kinetec chore-shell colums can be replace by new ChromShell colums. Just try it.

LUNA - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Luna Phenyl-Hexyl 3,5,10,15 100 400 17.5 1.5-10.0 L11
Luna Silica (2) 3,5,10,15 100 400 - - L3
Luna C5 5,10 100 440 12.5 1.5-10.0 -
Luna C8 5,10 100 440 14.75 1.5-10.0 L7
Luna C8 (2) 3,5,10,15 100 400 13.5 1.5-10.0 L7
Luna C18 5,10 100 440 19 1.5-10.0 L1
Luna C18 (2) 2.5,3,5,10,15 100 400 17.5 1.5-10.0 L1
Luna CN 3,5,10 100 400 7.0 1.5-10.0 L10
Luna NH2 3,5,10 100 400 9.5 1.5-11.0 L8
Luna SCX 5,10 100 400 0.55% Sulfur Load 2.0-7.0 L9
Luna HILIC 3,5 200 200 - 1.5-8.0 -
Luna PFP(2) 3 5 100 400 5.7 1.5-8.0 L43

GEMINI - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Gemini C18 3,5,10 110 375 14 1.0-12.0 L1
Gemini C6-Phenyl 3,5 110 375 12 1.0-12.0 L11
Gemini NX 3,5,10 110 375 14 1.0-12.0 L1

SYNERGI - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Synergi Max-RP 2.5 100 400 17 1.5-10.0 -
Synergi Hydro-RP 2.5 100 400 19 1.5-7.5 L1
Synergi Polar-RP 2.5 100 440 11 1.5-7.0 L11
Synergi Fusion-RP 2.5 100 440 12 1.5-10.0 L1
Synergi Max-RP 4,10 80 475 17 1.5-10.0 -
Synergi Hydro-RP 4,10 80 475 19 1.5-7.5 L1
Synergi Polar-RP 4,10 80 475 11 1.5-7.0 L11
Synergi Fusion-RP 4,10 80 475 12 1.5-10.0 L1

ONYX - PHENOMENEX

Packing Material Macropore Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Onyx Silica 2 130 300 0 2.0-7.5 -
Onyx C8 2 130 300 11 2.0-7.5 -
Onyx C18 2 130 300 18 2.0-7.5 -

JUPITER - PHENOMENEX

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Jupiter C4 5,10,15 300 170 5.0 1.5-10.0 L26
Jupiter C5 5,10,15 300 170 5.5 1.5-10.0 -
Jupiter C18 5,10,15 300 170 13.3 1.5-10.0 L1
Jupiter Proteo C12 4,10 90 475 15.0 1.5-10.0 -

GraceSmart - GRACE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
GraceSmart C18 3,5 120 220 10 2.0-9.0 L1

Alltech® Prevail - GRACE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
Prevail C18 3,5 110 350 17   L1
Prevail C18 Select 3,5 110 350 15   L1
Prevail C8 3,5 110 350 8   L7
Prevail Phenyl 3,5 110 350 7   L11
Prevail Cyano (CN) 3,5 110 350 -   L10
Prevail Amino (NH2) 3,5 110 350 -   L8
Prevail Silica 3,5 110 350 -   L3
Prevail Organic Acid 3,5 110 350 -   -
Carbohydrate ES (polymer) 5 - - -   -

Nano / Capillary LC Column ProteCol - SGE

Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Carbon Load (%) pH Range USP Packing
ProteCol C18 3 120/300 350 17 2.0-7.5 L1
ProteCol C8 3 120/300 350 10 2.0-7.5 L7
ProteCol C4 3 120/300 350   2.0-7.5 L26
ProteCol SCX 3 120/300 350   2.0-7.5 L9

 

 

 

 

 

 


Dosing pumps

Dosing pumps

Dosing pumps are used in many applications, in laboratories and within industry. Often we see the needs, when we require dose liquids under specific conditions:

  • High pressure dosing (reaction chambers, aparatures)
  • High temperatures dosing
  • Injection of reactive substances
  • Dosing of viscous media

Pump HeadFor all above mentioned applications it is possible to use technology that has been proven in the area of high performance liquid chromatography (HPLC). These are double-piston pumps AZURA (Knauer), which use sphire pistons enabling precise continuous dosing under high pressure. These pumps can work with flow of 0,01 to 1000 ml/min, under temperatures of -10°C to +120°C and with viscous media up to 1000 mPa.s.

The pumps can be especially equipped so they can be used in specific application like hazardous areas.

Nice example of use of AZURA pumps is dosing of sulphur trioxide in production of methanesulfonic acid (MSA).

Materials

Pump head are available in following materials:

  • Ceramic
  • Hastelloy C-276
  • Stainless steel
  • Titanum
  • Stainless steel/Titanum

Further information about models are available here.

 


TOPAZ Liners

SKY linersTrue Blue Performance

Exceptionally inert, Topaz™ inlet liners, with a new state-of-the-art deactivation,improve trace level analysis.

  • Increase accuracy and precision.
  • Lower detection limits.
  • Use wool with confidence.

When faced with complex choices, simple solutions stand out. TOPAZ™ inlet liners from Restek use a comprehensive, state-of-the-art deactivation and are the only blue liners on the market-making them an easy-to-recognize solution to common inlet problems.

The innovative deactivation used for TOPAZ™ liners results in exceptional inertness for a wide range of analyte chemistries. By reducing active sites and enhancing analyte transfer to the column, these liners increase accuracy and precision, allowing lower detection limits for many active compounds. In addition to improved data quality, you’ll benefit from fewer liner changes and less downtime for maintenance.

Selecting the right liner for your application can be a challenging task. TOPAZ™ inlet liners make the choice simple; the comprehensive deactivation, distinctive colour, and availability in popular configurations mean TOPAZ™ liners are the best choice for optimizing chromatographic performance. Regardless of your application, TOPAZ™ liners provide reliable inertness and assured performance, dayafter-day and analysis-after-analysis.


puriFlash RP

About Flash column stationary phases

Here you will find more information about stationary phases used in reversed phase Flash chromatography.

Reversed phases

puriFlRP-AQash® RP-AQ

60Å - 500 m2/g

15 & 30 μm

RP-alkyl, 6% Carbon

End-capping: mixed

pH stability: 2.0 to 7.5

Separation/purification of strongly and moderately polar molecules.

 

 


C18-AQ

puriFlash® C18-AQ

100Å - 300 m2/g

5, 10, 15 & 30 μm

C18 mono-functional, 14% Carbon

End-capping: mixed

pH stability: 2.0 to 7.5

Separation/purification of moderately polar and non-polar molecules.

 

 


C18-HP

puriFlash® C18-HP

100Å - 300 m2/g

5, 10, 15, 30 & 50 μm

C18 mono-functional, 16,5% Carbon

End-capping: one-step

pH stability: 1.5 to 7.5

This is an excellent choice for routine reverse phase purifications.

 

 

 


Uptisphere® Strategy™ C18-HQ

Uptisphere® Strategy™ C18-HQ

100Å - 425 m2/g

1.7, 2.2, 3, 5, 10, 15 μm

C18 mono-functional, 19% Carbon

End-capping: multi-step

pH stability: 1.0 to 10.0

Suitable for many pharmaceutical applications and routine methods.

 

 

 


puriFlash® C18-XS

puriFlash® C18-XS

100Å - 300 m2/g

5, 10, 3, 15 & 30 μm

C18 mono-functional, 17% Carbon

End-capping: multi-step

pH stability: 1.0 to 10.0

It is an excellent phase for the complete separation of basic molecules.

 

 

 


There are mor phases available. Contact us for more information for suitable stationary phase for Flahs/Purification chromatography.

 

 


puriFlash

puriFlash® Flash Cartridges

Interchim developed new technique for flash chromatography - Ultra Performance Flash Purification (UPFP) using special flash cartridges. The flash cartridges are in the form of regular or irregular silica. UPFP enables to run purifications with high purity of the yield and less solvent use. 

puriFlash cartridges

Flash column selection

Flash column celection is available at this page.


Stationary phases

Chromatography phasesChromatography, either GC, HPLC, SPE, FLASH or preparative, uses many types of stationary phases. Here you wil find detailed information about stationary phases.

Stationary phases for analytical separation

Stationary phases for sample preparation

  • SPE
  • dSPE (QuEChERS)
  • IAC (Immunoaffinity Columns)
  • MEPS (Micro Extraction by Packed Sorbent)
  • FLASH
  • BULK (media for preparative chromatography)

 


Preparative LC

External flow cellTask for preparative HPLC systems differs to analytical one. While analytical HPLC task is qualitative and quantitative determination of defined compounds in samples, preparative HPLC task is separation, purification and isolation of value products from mixtures.

Preparative chromatography can be devidet into three main areas:

  • Semi-preparative separations
  • Batch preparative chromatography (pilot or industrial scale)
  • True Counter-current chromatography
  • Simulated moving bed (SMB)
  • Continuous chromatography

Scale definition

Parameter Analytical Semi-preparative Preparative
Column sizes (mm) 120 - 250 x 2 - 4.6 120 - 250 x 8 - 16 120 - 250 x 20 - 62
Particle size (µm) up to 5 5 - 10 higher than 10
Stationary phase (g) up to 5 5 - 30 50 - 450
Tubings 1/16" 1/16" 1/8"
Flow rates (ml/min) 0.1 - 2 5 - 50 100 - 1000
Sample size (mg) 0.01 - 2 0.1 - 50 1 - 700
Flow cell (mm) 10 3 0.5 - 2

How the Triple Quadrupole works?

EVOQ Triple QuadrupoleThe principle of Triple Quadrupole (TQ) is explained on EVOQ™ system by Bruker.  The key points of the system are:

  • Axial Ion Source
  • Active Focusing Q1
  • Lens-Free Ion Path
  • 180° Collision Cell
  • Elliptical Design
  • Off Axis Detector

 Look at the video to understand how the Triple quadrupole system works:


UHPLC

UHPLC PLATINblueUltra-High Performance Liquid Chromatography a milestone in the evolution of LC in that columns packed with <2µm particles, used with instrumentation capable of handling the resulting high back pressures, make possible extremely fast and efficient separations. UHPLC is a very powerful tool for today’s practicing chromatographer, as it can significantly increase the efficiency of a chromatographic separation. In addition, the wider range of usable flow rates makes high speed separations possible.

Effect on efficience

The lower the particle size, the higher column efficience is (see graph below). With particle size decrease the column back pressure increases significantly. This leads to ultra high pressure of longer LC columns. It means that we cannot use 1,9µm UHPLC column the same lenth like 5µm column (eg. 250 mm long column). In the end we have lower or similar column efficieny as we have with standard HPLC columns. The main benefit of the UHPLC columns is the analysis run time, not the efficiency.

Comparison of Particle Size Efficiences

If you need to increase efficiency, look at phase chemistry first. In this case look at stationary phase list.


UHPLC Reagents

UHPLC solventsThe UHPLC technology requires far better quality solvents than what is currently available on the market. Biosolve ULC/MS solvents, buffers and acids line combine the highest demands for:

  • UV low gradient drift
  • Minimal peak impurities
  • Lowest ionic background in MS detection
  • Less than 100 ppb of Alkali metal

ULC/MS solvents, are micro filtered at 0,1 µm, have a residue after evaporation of max. 1 ppm and are packed under inert gas for better shelf-life. Besides the standard 2,5 l packaging, Biosolve is now offering for nano LC/MS:

  • 500 ml bottles of acetonitrile, methanol, and isopropanol
  • 1 l bottle of water
  • 100 ml TFA

Information about the reagencies are available. Just ask for it.


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