Dosing pumps are used in many applications, in laboratories and within industry. Often we see the needs, when we require dose liquids under specific conditions:
High pressure dosing (reaction chambers, aparatures)
High temperatures dosing
Injection of reactive substances
Dosing of viscous media
For all above mentioned applications it is possible to use technology that has been proven in the area of high performance liquid chromatography (HPLC). These are double-piston pumps AZURA (Knauer), which use sphire pistons enabling precise continuous dosing under high pressure. These pumps can work with flow of 0,01 to 1000 ml/min, under temperatures of -10°C to +120°C and with viscous media up to 1000 mPa.s.
The pumps can be especially equipped so they can be used in specific application like hazardous areas.
Nice example of use of AZURA pumps is dosing of sulphur trioxide in production of methanesulfonic acid (MSA).
Pump head are available in following materials:
Further information about models are available here.
Exceptionally inert, Topaz™ inlet liners, with a new state-of-the-art deactivation,improve trace level analysis.
Increase accuracy and precision.
Lower detection limits.
Use wool with confidence.
When faced with complex choices, simple solutions stand out. TOPAZ™ inlet liners from Restek use a comprehensive, state-of-the-art deactivation and are the only blue liners on the market-making them an easy-to-recognize solution to common inlet problems.
The innovative deactivation used for TOPAZ™ liners results in exceptional inertness for a wide range of analyte chemistries. By reducing active sites and enhancing analyte transfer to the column, these liners increase accuracy and precision, allowing lower detection limits for many active compounds. In addition to improved data quality, you’ll benefit from fewer liner changes and less downtime for maintenance.
Selecting the right liner for your application can be a challenging task. TOPAZ™ inlet liners make the choice simple; the comprehensive deactivation, distinctive colour, and availability in popular configurations mean TOPAZ™ liners are the best choice for optimizing chromatographic performance. Regardless of your application, TOPAZ™ liners provide reliable inertness and assured performance, dayafter-day and analysis-after-analysis.
Interchim developed new technique for flash chromatography - Ultra Performance Flash Purification (UPFP) using special flash cartridges. The flash cartridges are in the form of regular or irregular silica. UPFP enables to run purifications with high purity of the yield and less solvent use.
Task for preparative HPLC systems differs to analytical one. While analytical HPLC task is qualitative and quantitative determination of defined compounds in samples, preparative HPLC task is separation, purification and isolation of value products from mixtures.
Preparative chromatography can be devidet into three main areas:
Batch preparative chromatography (pilot or industrial scale)
Ultra-High Performance Liquid Chromatography a milestone in the evolution of LC in that columns packed with <2µm particles, used with instrumentation capable of handling the resulting high back pressures, make possible extremely fast and efficient separations. UHPLC is a very powerful tool for today’s practicing chromatographer, as it can significantly increase the efficiency of a chromatographic separation. In addition, the wider range of usable flow rates makes high speed separations possible.
The lower the particle size, the higher column efficience is (see graph below). With particle size decrease the column back pressure increases significantly. This leads to ultra high pressure of longer LC columns. It means that we cannot use 1,9µm UHPLC column the same lenth like 5µm column (eg. 250 mm long column). In the end we have lower or similar column efficieny as we have with standard HPLC columns. The main benefit of the UHPLC columns is the analysis run time, not the efficiency.
If you need to increase efficiency, look at phase chemistry first. In this case look at stationary phase list.
The UHPLC technology requires far better quality solvents than what is currently available on the market. Biosolve ULC/MS solvents, buffers and acids line combine the highest demands for:
UV low gradient drift
Minimal peak impurities
Lowest ionic background in MS detection
Less than 100 ppb of Alkali metal
ULC/MS solvents, are micro filtered at 0,1 µm, have a residue after evaporation of max. 1 ppm and are packed under inert gas for better shelf-life. Besides the standard 2,5 l packaging, Biosolve is now offering for nano LC/MS:
500 ml bottles of acetonitrile, methanol, and isopropanol
1 l bottle of water
100 ml TFA
Information about the reagencies are available. Just ask for it.