Check gas purifiers and if they are spent, replace them.
Clean injector and detector
Replace injector/detector liners with new ones.
Replace critical injector and detector seals.
Replace septum in the injector.
Set make-up and detector gas flow rates.
Carefully inspect the column for damage or breakage.
Install a nut and ferrule on each end of the column.
Cut 10 centimeters from each end of the column. Use a sapphire scribe or ceramic scoring wafer to cut fused silica capillary columns. Use the serrated edge of a ceramic scoring wafer or the edge of a sharp file to cut metal capillary columns. See our catalogue for capillary cuting tools.
Mount the capillary column in the oven using a bracket that protects the column from becoming scratched or abraded.
Insert column the appropriate distance into the inlet as indicated in the instrument manual.
Install the column so that the capillary does not touch the oven walls.
Set the approximate column flow rate by adjusting the head pressure to the value listed on the test chromatogram included with the column.
Set split vent, septa purge, and any other applicable inlet gases according to the instrument specifications.
Confirm the flow by immersing the column outlet in a vial of solvent (acetone or isopropyl alcohol).
Insert column the appropriate distance into the detector as indicated in the instrument manual.
Check for inlet and outlet leaks using a thermal conductivity leak detector. Do not use soaps or liquid-based leak detectors or the column may be damaged.
Set injector and detector temperatures. Turn the detector on when the temperatures have equilibrated. Caution - do not exceed the phase's maximum operating temperature!
To set the proper dead time (linear velocity), inject methane or a non-retained substance compatible with the detector being used.
Verify system integrity by checking the dead volume peak. It should not tail.
Condition the column at its maximum operating temperature to stabilize the baseline. (See the test chromatogram included with the column for the maximum temperature.)
Set oven to appropriate temperature and inject methane or an appropriate unretained substance, again to set the proper linear velocity.
Inject a duplicate of the original test mixture or your specific test mixture to confirm proper installation, system, and column performance.