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Task for preparative HPLC systems differs to analytical one. While analytical HPLC task is qualitative and quantitative determination of defined compounds in samples, preparative HPLC task is separation, purification and isolation of value products from mixtures.
Preparative chromatography can be devidet into three main areas:
Parameter | Analytical | Semi-preparative | Preparative |
---|---|---|---|
Column sizes (mm) | 120 - 250 x 2 - 4.6 | 120 - 250 x 8 - 16 | 120 - 250 x 20 - 62 |
Particle size (µm) | up to 5 | 5 - 10 | higher than 10 |
Stationary phase (g) | up to 5 | 5 - 30 | 50 - 450 |
Tubings | 1/16" | 1/16" | 1/8" |
Flow rates (ml/min) | 0.1 - 2 | 5 - 50 | 100 - 1000 |
Sample size (mg) | 0.01 - 2 | 0.1 - 50 | 1 - 700 |
Flow cell (mm) | 10 | 3 | 0.5 - 2 |