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  1. Chromservis
  2. Chromatography
  3. Flash and Preparative HPLC
  4. Preparative LC
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Chromatography

Flash and Preparative HPLCGC/MS-TOF
    GC/MS/MS
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        Preparative LC

        External flow cellTask for preparative HPLC systems differs to analytical one. While analytical HPLC task is qualitative and quantitative determination of defined compounds in samples, preparative HPLC task is separation, purification and isolation of value products from mixtures.

        Preparative chromatography can be devidet into three main areas:

        • Semi-preparative separations
        • Batch preparative chromatography (pilot or industrial scale)
        • True Counter-current chromatography
        • Simulated moving bed (SMB)
        • Continuous chromatography

        Scale definition

        Parameter Analytical Semi-preparative Preparative
        Column sizes (mm) 120 - 250 x 2 - 4.6 120 - 250 x 8 - 16 120 - 250 x 20 - 62
        Particle size (µm) up to 5 5 - 10 higher than 10
        Stationary phase (g) up to 5 5 - 30 50 - 450
        Tubings 1/16" 1/16" 1/8"
        Flow rates (ml/min) 0.1 - 2 5 - 50 100 - 1000
        Sample size (mg) 0.01 - 2 0.1 - 50 1 - 700
        Flow cell (mm) 10 3 0.5 - 2

        Preparative chromatography can be conbined with Flash chromatography in one system - purification device. The PuriFlash (Advion-Interchim) offers different modes of operation:

        • Preparative line + Flash line
        • Two Flash lines
        • Two Preparative lines.
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