
MetAmino® kits offer an easy sample preparation method for your LC-MS or GC-MS analysis. MetAmino® kits incude derivatization reagences and all clean-up accessories to prepare your sample for injection. They elimilate the time consuming sample preparation procedures.
New clean-up procedure uses a special material as the end-step. The other odvantage is that the derivatization procedure enables to extenbd the analyte list.
4015 kB
Sorbents for the MSPE technique are chosen to cover the widest possible field of applications. MSPE SpeExtra C18 is a hydrophobic type of octadecyl silica gel with a special endcapping. It is suitable for a wide range of analytes, showing lower retention for polar compounds. MSPE SpeExtra C18-P is a polar modified monomeric octadecyl silica gel. It offers different types of interactions: dipole-dipole, π-π and hydrophobic. It is therefore suitable for aromatic and polar compounds. MSPE SpeExtra HLB polymer sorbent with high specific surface area and special endcapping. It has a hydrophilic and lipophilic modification ensuring universal use and a higher capacity than C18 silica gel.
MSPE sorbent | Particle size [µm] | Specific surface area [m 2 /g] | ||
C18 | 60 | 310 | ||
C18-P | 60 | 310 | ||
HLB | 30 | 850 |
Hemp contains hundreds of cannabinoids with Cannabidiol (CBD) being the most prevalent in the plant and Δ9-Tetrahydrocannabinol (THC) being the active ingredient causing psychotropic effects. However, many more compounds are formed by the hemp plant and have been investigated for their medical effects. This limit often requires THC remediation of the distilled hemp extract (starting material) and can be achieved using preparative scale chromatography such as the puriFlashR XL-Cannabis system. HPLC analysis of the starting material (third pass distillate), fractions collected during the remediation process, and the finished product can be performed using the Advion AVANT HPLC-UV analytical system. Both the purification and analytical processes are shown in this application note to form a complete solution for THC remediation in the hemp industry.
This site encloses SPE phases overview includint their technical parameters. Further information about the products are available in the product catalogue.
Phase | Pore volume (cm3/g) | Pore Size (A) | Surface Area (m2/g) | Carbon Load (%) | End Capping | Feature |
---|---|---|---|---|---|---|
C2 Ethyl | 0.77 | 60 | 500 | 6.6 | YES/NO | |
C4 n-Butyl | 0.77 | 60 | 500 | 8.5 | YES | |
C8 Octyl | 0.77 | 60 | 500 | 11.1 | YES/NO | |
C18 Octadecyl | 0.77 | 60 | 500 | 21.7 | YES/NO | |
C30 Tricontyl | 0.77 | 60 | 500 | 20.0 | YES | |
Cyclohexyl | 0.77 | 60 | 500 | 11.6 | YES/NO | |
Phenyl | 0.77 | 60 | 500 | 11.0 | YES/NO |
Phase | Pore volume (cm3/g) | Pore Size (A) | Surface Area (m2/g) | Carbon Load (%) | Feature | |
---|---|---|---|---|---|---|
Silica | 0.77 | 60 | 500 | N/A | ||
Diol | 0.99 | 60 | 500 | 8.0 | ||
Cyanopropyl | 0.77 | 60 | 500 | 9.0 | ||
Florisil | 0.82 | 60 | 500 | N/A | ||
Alumina, Acidic | 60 | 500 | N/A | |||
Alumina, Basic | 60 | 500 | N/A | |||
Alumina, Neutral | 60 | 500 | N/A | |||
Carbon | N/A | 120/140 mesh |
Phase | Pore volume (cm3/g) | pKa | Pore Size (A) | Surface Area (m2/g) | Carbon Load (%) | Exchange (meq/g) |
---|---|---|---|---|---|---|
Aminopropyl (1 amine) | 0.77 | 9.8 | 60 | 500 | 6.65 | 0.31 |
N-2 Aminoethyl (1/2 amine) | 0.77 | 10.1; 10.9 | 60 | 500 | 11.1 | 0.32 |
Diethylamino (3 amine) | 0.77 | 10.6 | 60 | 500 | 10.6 | 0.28 |
Quarternary Amine Chloride | 0.77 | Always charged | 60 | 500 | 8.4 | 0.25 |
Quarternary Amine Hydroxide | 0.77 | Always charged | 60 | 500 | 8.4 | 0.25 |
Quarternary Amine Acetate | 0.77 | Always charged | 60 | 500 | 8.4 | 0.25 |
Quarternary Amine Formate | 0.77 | Always charged | 60 | 500 | 8.4 | 0.25 |
Polyimine | 0.77 | Always charged | 13.5 | 0.25 |
Phase | Pore volume (cm3/g) | pKa | Pore Size (A) | Surface Area (m2/g) | Carbon Load (%) | Exchange (meq/g) |
---|---|---|---|---|---|---|
Carboxylic Acid | 0.77 | 4.8 | 60 | 500 | 9.2 | 0.17 |
Propylsulfonic Acid | 0.77 | 1 | 60 | 500 | 7.1 | 0.18 |
Benzenesulfonic Acid | 0.77 | Always charged | 60 | 500 | 11.0 | 0.32 |
Benzenesulfonic Acid, High Load | 0.77 | Always charged | 60 | 500 | 15.0 | 0.65 |
Triacetic Acid | 0.77 | 60 | 500 | 7.61 | Anion 0.17/Cation 0.06 |
Phase | Pore volume (cm3/g) | pKa | Pore Size (A) | Surface Area (m2/g) | Carbon Load (%) | Exchange (meq/g) |
---|---|---|---|---|---|---|
Aminopropyl + C8 | 0.77 | 9,8 | 60 | 500 | 12,3 | 0,163 |
Quarternary Amine + C8 | 0.77 | Always charged | 60 | 500 | 13,6 | 0,160 |
Carboxylic Acid + C8 | 0.77 | 4,8 | 60 | 500 | 2,5 | 0,105 |
Propylsulfonic Acid + C8 | 0.77 | 1 | 60 | 500 | 14,62 | 0,114 |
Benzenesulfonic Acid + C8 | 0,77 | Always charged | 60 | 500 | 12.3 | 0,072 |
Cyanopropyl + C8 | 0,77 | N/A | 60 | 500 | 14,6 | 0,163 |
Cyclohexyl + C8 | 0.77 | N/A | 60 | 500 | N/A | N/A |
On this page we provide an overview of the supplied stationary phases for gas chromatography (GC). Each is given details of its properties and the applications that are suitable for them. In the product catalog you can then choose a suitable quartz or metal capillary column for GC.
Stationary phase | Temperature range | Composition | USP Phase |
---|---|---|---|
LN-1 | -60 to 370°C | 100% dimethyl polysiloxane | G2 |
LN-1 MS | -60 to 370°C | 100% dimethyl polysiloxane | G2 |
LN-1 HT | -60 to 430°C | 100% dimethyl polysiloxane | - |
LN-5 | -60 to 370°C | 5% diphenyl/95% dimethyl polysiloxane | G27 |
LN-5 Sil MS | -60 to 370°C | 5% diphenyl/95% dimethyl polysiloxane | G27 |
LN-5 MS | -60 to 350°C | 5% phenyl - arylene - 95% dimethyl polysiloxane | G27 |
LN-5 HT | -60 to 430°C | 5% diphenyl/95% dimethyl polysiloxane | - |
LN-35 | 50 to 360°C | 35% diphenyl/65% dimethyl polysiloxane | G42 |
LN-35 HT | -60 to 400°C | 35% diphenyl/65% dimethyl polysiloxane | G42 |
LN-17 | 40 to 340°C | 50% diphenyl/50% dimethyl polysiloxane | G3 |
LN-624 | -20 to 260°C | 6% cyanopropylphenyl/94% dimethyl polysiloxane | G43 |
LN-FFAP | 40 to 260°C | Nitroterephthalic Acid Modified Polyethylene Glycol | G35 |
LN-1701 | -20 to 300°C | 14% cyanopropylphenyl/86% dimethyl polysiloxane | G46 |
LN-XLB | 30 to 360°C | Low polarity phases | - |
LN-XLB-HT | 30 až 400°C | Low polarity phases | |
LN-WAX | 40 to 260°C | Polyethylene Glycol | G16 |
LN-WAX Plus | 20 to 260°C | Polyethylene Glycol | G16 |
Ultra-High Performance Liquid Chromatography a milestone in the evolution of LC in that columns packed with <2µm particles, used with instrumentation capable of handling the resulting high back pressures, make possible extremely fast and efficient separations. UHPLC is a very powerful tool for today’s practicing chromatographer, as it can significantly increase the efficiency of a chromatographic separation. In addition, the wider range of usable flow rates makes high speed separations possible.
The lower the particle size, the higher column efficience is (see graph below). With particle size decrease the column back pressure increases significantly. This leads to ultra high pressure of longer LC columns. It means that we cannot use 1,9µm UHPLC column the same lenth like 5µm column (eg. 250 mm long column). In the end we have lower or similar column efficieny as we have with standard HPLC columns. The main benefit of the UHPLC columns is the analysis run time, not the efficiency.
If you need to increase efficiency, look at phase chemistry first. In this case look at stationary phase list.
Packing Material | Particle Size (µm) | Pore Size (Å) | Effective Surface Area (m2/g) | Carbon Load (%) | pH Range |
---|---|---|---|---|---|
Raptor ARC-C18 | 1.8 | 90 | 125 | proprietary | 1.0-8.0 |
Raptor ARC-C18 | 2.7 | 90 | 130 | proprietary | 1.0-8.0 |
Raptor ARC-C18 | 5.0 | 90 | 100 | proprietary | 1.0-8.0 |
Raptor C18 | 1.8 | 90 | 125 | proprietary | 2.0-8.0 |
Raptor C18 | 2.7 | 90 | 130 | proprietary | 2.0-8.0 |
Raptor C18 | 5.0 | 90 | 100 | proprietary | 2.0-8.0 |
Raptor Biphenyl | 1.8 | 90 | 125 | proprietary | 1.5-8.0 |
Raptor Biphenyl | 2.7 | 90 | 130 | proprietary | 1.5-8.0 |
Raptor Biphenyl | 5.0 | 90 | 100 | proprietary | 1.5-8.0 |
Raptor Fluorophenyl | 1.8 | 90 | 125 | proprietary | 2.0-8.0 |
Raptor Fluorophenyl | 2.7 | 90 | 130 | proprietary | 2.0-8.0 |
Raptor Fluorophenyl | 5.0 | 90 | 100 | proprietary | 2.0-8.0 |
Raptor HILIC-Si | 2.7 | 90 | 150 | n/a | 2.0-8.0 |
Raptor EtG/EtS | 2.7 | 90 | 130 | proprietary | 2.0-8.0 |
Raptor maximum pressure: 1,034 bar (1.8 μm), 600 bar (2.7 μm); 400 bar (5 μm). For maximum lifetime recommended maximum pressure for 1.8 µm particles is 830 bar.
Packing Material | Particle Size (µm) | Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | pH Range |
---|---|---|---|---|---|
Pinnacle DB C18 | 1.9 | 140 | 150 | 11 | 2.5-8.0 |
Pinnacle DB Aqueous C18 | 1.9 | 140 | 150 | 6 | 2.5-8.0 |
Pinnacle DB C8 | 1.9 | 140 | 150 | 6 | 2.5-8.0 |
Pinnacle DB CN | 1.9 | 140 | 150 | 4 | 2.5-8.0 |
Pinnacle DB PFP | 1.9 | 140 | 150 | 6 | 2.5-8.0 |
Pinnacle DB Biphenyl | 1.9 | 140 | 150 | 8 | 2.5-8.0 |
Pinnacle DB IBD | 1.9 | 140 | 150 | proprietary | 2.5-8.0 |
Pinnacle DB Silica | 1.9 | 140 | 150 | n/a | 2.5-8.0 |
Many GC and LC problems can be avoided with routine preventive maintenance. If you are seeking the cause of the chromatography problem, go step by step. Never make more changes in your instrument at the same time, otherwise you will never find, what caused the problem.
Select your category of chromatography to read more about the troubleshooting: (GC troubleshooting is not available yet)
Interchim developed new technique for flash chromatography - Ultra Performance Flash Purification (UPFP) using special flash cartridges. The flash cartridges are in the form of regular or irregular silica. UPFP enables to run purifications with high purity of the yield and less solvent use.
Flash column celection is available at this page.