Chromatography / Hints and tips

TOPAZ Liners

SKY linersTrue Blue Performance

Exceptionally inert, Topaz™ inlet liners, with a new state-of-the-art deactivation,improve trace level analysis.

  • Increase accuracy and precision.
  • Lower detection limits.
  • Use wool with confidence.

When faced with complex choices, simple solutions stand out. TOPAZ™ inlet liners from Restek use a comprehensive, state-of-the-art deactivation and are the only blue liners on the market-making them an easy-to-recognize solution to common inlet problems.

The innovative deactivation used for TOPAZ™ liners results in exceptional inertness for a wide range of analyte chemistries. By reducing active sites and enhancing analyte transfer to the column, these liners increase accuracy and precision, allowing lower detection limits for many active compounds. In addition to improved data quality, you’ll benefit from fewer liner changes and less downtime for maintenance.

Selecting the right liner for your application can be a challenging task. TOPAZ™ inlet liners make the choice simple; the comprehensive deactivation, distinctive colour, and availability in popular configurations mean TOPAZ™ liners are the best choice for optimizing chromatographic performance. Regardless of your application, TOPAZ™ liners provide reliable inertness and assured performance, dayafter-day and analysis-after-analysis.

Čištění injektoru


  • Udává se, že příčinu 85-90% problémů při analýze lze najít v inletu. Proto nezapomínejte pravidelně vyměňovat všechen spotřební materiál. Liner, septum i veškerá těsnění mají omezenou životnost!
  • Někdy však výměna spotřebního materiálu ani zaříznutí kolony nestačí. Pak je nutno vyčistit inlet. Obecné pokyny naleznete níže, ale vždy se řiďte především pokyny svého výrobce!



  • Inlet zchlaďte. Teplota by neměla přesahovat 40°C.
  • Vypněte průtok nosného plynu.
  • Deinstalujte případný autosampler.
  • Deinstalujte kolonu.
  • Otevřete inlet, vyjměte veškerý spotřební materiál.
  • Pokud lze, je vhodnější odpojit splitovou větev pneumatického systému od inletu.
  • Inlet nyní sestává pouze z kovové trubky, která může a nemusí být na konci zúžená.
  • Existují různé nástroje, které lze použít k čištění (např. Restek). Pomocí takového kartáče a rozpouštědla- methylen chlorid a metanol pohyby dolů a vzhůru vyčistěte inlet.
  • Pomocí pipety prostříkněte inlet rozpouštědlem (rozpouštědlo pod inletem zachyťte do kádinky) a ujistěte se, že v inletu nezůstaly žádné částečky nečistot.
  • Pro odstranění zbytků rozpouštědla nahřejte inlet na cca 65 °C.
  • Reinstalujte splitovou větev pneumatického systému, nainstalujte nový spotřební materiál.
  • Zapněte průtok nosného plynu. Zkontrolujte těsnost.
  • Před zvýšením teploty nechte inlet alespoň 10minut proplachovat. Odstraníte tak zbytky kyslíku. Předčasným zvýšením teploty může dojít k aktivaci a znehodnocení nového spotřebního materiálu.

Cleaning the detector


Audible noise, random ghost peaks, low sensitivity. These are typical characteristics of dirty FID detector.

The most common cause contamination sulfide is bleeding from the column. Burned stationary phase may be deposited on the nozzle surface of the detector and cause problems. However, the nozzle napalují and other contaminants.

You need to clean your detector?

The above-described problems, however, may not only be caused by contamination of the detector. The steps outlined below will help rule out other potential causes.

The carrier gas and the stationary phase bleeding

Possible source of contamination can be found not only in the detector itself, but also in front of him. Bleeding stationary phase column, septum, inlet contaminated, contaminated carrier gas ... To eliminate this source Blind FIDU corresponding input plug and turn the FID. If the problem ceases, search problem outside detector. No need to replace the liner? Septum? Clean inlet? What is the state column? Do you have a pure carrier gas? You do not have a leak in the system?

Hydrogen and air

Even hydrogen and air used in the sulfide can be a source of contamination. Attention: especially when problems emerged after replacing the cylinder.

Also improper flow / pressure of the two gases can be a source of increased noise and reduced sensitivity to ignition problems sulfide. Make flows through the meter.

Electrical System

I electrical interference may exhibit similar symptoms dirty FID. There may be a defect electrometer, poor contact or interference by other devices in the lab.

Before cleaning

  • Make sure to unplug the power cord!
  • Remember that the detector may be hot!
  • When dismantling FIDU pay attention insulating parts. Use tweezers, whether these parts do not transfer dirt from your hands or gloves. Beware of possible scratches.
  • Remember that sometimes it may be easier to change the nozzle before it is cleaned. This is particularly true when the nozzle is heavily contaminated and sharply increases the risk of scratches during nozzle cleaning.


  • Remove the nozzle from the sulfide.
  • Place it in an ultrasonic bath with water and detergent and ultrazvukujte about 5-10min.
  • Use a tool or a suitable thin wire purge nozzle. Be careful. Any scratches may change the shape of the flame, increasing noise or loss of sensitivity.
  • Re-insert the nozzle into the ultrasonic bath and ultrazvukujte other 5-10min. From now handling the nozzle use only tweezers.
  • Rinse the nozzle with clean water.
  • Rinse nozzle small amount of methanol.
  • Blow nozzle stream of air or nitrogen.
  • Let dry nozzle.
  • Seskládejte FID. Pay attention to tightening. When you drag nozzle may cause its deformation!
  • After seskládání can connect column. It is appropriate to heat the FID temperature 10 ° C-40 ° C higher than the normal operating temperature of the detector. Note maximum temperature limit FIDU! Note the maximum operating temperature of the column!

How to keep your detector FIT

  • A new column is bleeding most. Install the column and into the inlet, as usual, but let the detector end freely in the furnace and condition the column. Then install the column and into the detector.
  • Use a good quality, low column bleed.
  • Moisture and oxygen in the carrier gas deplete the stationary phase of the column and cause a bleeding. Wear high-purity gases, molecular sieves, traps ... Check the tightness of the gas circuit.
  • Use appropriate septa with low bleed and change them often enough.


ECD is a specific and sensitive detector. Inappropriate behavior, however, can sharply reduce its lifespan. The gradual increase in signal at this detector normal. However, if an increase occurs abruptly or adding more of the symptoms worsening-noise desensitization search problem.


  • ECD contains radioactive material, therefore, mandated regular wear tests. It is forbidden to open or to interfere in any way.
  • ECD can be cleaned only thermally. Generally, cleaning is performed so that the ECD detector heated to a temperature close to its maximum operating temperature and impurities are burned. Before cleaning, be sure there are no leaks. Raising the temperature takes place gradually. Track signal. Increase the temperature by 10-20 ° C, the signal starts to grow. Wait for the signal to stabilize and begin to decline, then you can again increase the temperature. Upon reaching the desired temperature, wait for the signal to decrease the expected values.
  • Follow the instructions of your manufacturer, the procedure may vary.
  • If you heat detector installed column exceed the maximum operating temperature of the column or column Deinstall detector and replace the plug.

Do not ruin your detector!

  • Use good quality gas intended for ECD.
  • Use molecular sieves, traps for purification of gases.
  • When potížích- increase in signal noise worse, desensitizing like. Check system for leaks.
  • Use quality columns and septa with low bleeding.

Syringe tip selection

The selection of suitable syring tip is mentioned in Hamilton catalogue. Please, ask for this catalogue.

GC troubleshooting


Many GC problems can be prevented if the column is properly installed and GC is maintained routinely. For example, replacing septa or liner at regular intervals and keeping the injector and detector clean and well-maintained shoud solve many problems. Regular preventive maintenance depends on particular model of GC and you should consult required operations in the operator's and service manuals.

For the problem identification we recommend to use electronic flowmeter and leak detector.

Baseline problems

problemBaseline problems could be divided into 5 categories: drift, noise, offset, spiking and wander.

  • Drift means slow baseline movement in one direction
  • Noise is rapid and random movement of the baseline position
  • Offset is sudden unexplained change of the baseline position
  • Spiking is presented by peaks with no width, either positive or negative
  • Wander is low frequency noise
Downward drift
Possible cause Suggestions
Downward drift for a few minutes is normal after installing a new column Increase the oven temperature to close to the maximum continuous operating temperature for the column. Maintain the temperature until flat baseline is observed. If the detector signal does not drop in 10 minutes, immediately cool the column and check for leaks.
Unequilibrated detector Allow sufficient time for temperature equilibration of the detector.
Downward drift is frequently due to the "back-out" of contaminants from the detector or other parts of the GC Clean out contamination.
Upward drift
Possible cause Suggestions
Damage to the stationary phase of the GC column Determine the cause of the damage. It may be due to impurities in the carrier gas or to excessive temperatures. Replace column.
Drift in gas flow rates Clean or replace flow or pressure regulator(s). Adjust pressure.
Possible cause Suggestions
The column may be inserted too far into the flame of an FID, NPD or FPD detector Reinstall the column. Be sure to insert the column into the detector exactly the correct distance specified in the instrument manual.
An air leak can result in noise in ECD and TCD detectors Eliminate the leak.
Incorrect combustion gases or flow rates can generate nois in FID, NPD or FPD detectors. Be sure yuor gases are the proper grade, as well as clean and dry. Reset the flow rates of the gases to their proper values.
Contaminated injector Clean injector. Replace inlet liner, septa and selas.
Contaminated column Bake out the column. Cut off first 10 cm of column. If it does not help, replace the column.
Defective detector Clean and/or replace parts as necessary.
Defective detector board Consult GC manufacturer.
Possible cause Suggestions
Line voltage changes Monitor line voltage for correlation with offset. If correlation is found, install voltage regulator or ensure stable power supply.
Poor electrical changes Check electrical connections. Tighten any loose connections. Clean any dirty or corroded connections.
Contaminated injector Clean injector. Replace inlet liner, septa and selas.
Contaminated column Bake out the column. Cut off first 10 cm of column. If it does not help, replace the column.
Column inserted too fat into the flame of FID, NPD or FPD detectors Reinstall the column. Be sure to insert the column into the detector exactly the correct distance specified in the instrument manual.
Contaminated detector Clean the detector if possible.
Possible cause Suggestions
Electrical disturbances entering the chromatograph through power cables, even shielded cables Try to correlate spikes with events in equipment near the chromatograph. Periodicity is often a clue. Turn off equipment or move it. If necessary, install a voltage regulator.
Possible cause Suggestions
Baseline wandering may be caused by changes in environmental conditions such as temperature or line voltage Try to correlate the wandering with environmental parameters. If a correlation is observed, you will know what to do.
Inadequate temperature control Check if variations can be correlated with changes in the baseline position. Measure detector temperature.
Wandering while using isothermal conditions may be due to contaminated carrier gas Change the carrier gas or the gas purification traps.
Contaminated injector Clean injector. Replace inlet liner, glass wool and seals.
Contaminated column Bake out the column. Cut off first 10 cm of column. If it does not help, replace the column.

Distorted peak shapes

stabilityAll peaks redused in size
Possible cause Suggestions
Sample validity Check the concentration and stability of the sample.



Flattened top peaks
Possible cause Suggestions
Detector overload. The broad peaks may have a rounded top or even valleys in the top. Reduce sample volume, dilute with solvent or use higher split ratio.
Overload of the signal processing electronics. The peaks are clipped with flat tops. Attenuate detector output or reduce sample amount (see above).
Fronting peaks

Fronting peaks are usually the result of column overloading. In this case, the effect should be a function of injection volume. Solutions include reducing the injection volume or using a column with greatr capacity. Columns with larger diameter or thicker stationary phase coatings generally have larger sample capacities, however, resolution may be reduced.

Ghost peaks
Possible cause Suggestions
Remmants of previous samples in the inlet or column. Ghost peaks due to remmants are most likely to occur when increasing inlet or column temperatures. Increase the final temperature and lengthen the run time to allow for complete elution of previous samples. If ghost peaks continue to occur, clean the inlet. Condition the column at temperature higher than has been used but lower than the maximum continuous operation temperature for the column. Cut 10 cm off the inlet end of the column and/orreverese it before reconditioning it. If it does not help, replace the column.
Backflash may cause remmants. Backflash refers to vapours from the sample which expand to exceed the volume of the injector liner. These vapours may come in contact with colder spots, such as the septum and gas inlets of the injector. Less volatile components may condense. These condensates may vaporize later and interfere with subsequent analyses, sometimes producing "ghost peaks".
  • Use septum purge
  • Lower injection volume
  • Enlarge injector liner
  • Optimize injector temperature
  • Use pressure pulsed program


Bleed from the septum or fragments of the septum lodged in the inlet or liner. Clean the inlet. Replace the inlet liner, glass wool and seals.
Irreproducible peak heights or areas
Possible cause Suggestions
Inconsistent injection Develop a reproducible injection technique. Use autosampler.
Distorted peak shapes can adversely affect quantitative determinations Correct any problems that result in the distortion of peak shape. See Peak shape problems.
Baseline disturbances See Baseline problems.
Variations in GC operating parameters Standardize operating parameters.
Negative peaks
Possible cause Suggestions
Incorrect polarity of the recorder Reverse polarity of recorder connections.
Incorrect setup in the software Set-up right parameters in your chromatography software.
Sample compound has greater thermal conductivity than the carrier gas and you are using a TCD or µTCD detector If possible, change carrier gas. Otherwise there is not a solution.
Detector overload in element-specific detectors such as ECD, NPD, FPD, etc., can produce both positive and negative peaks Have the compound of interest arrive at the detector at a different time from the solvent or other compounds in high concentration. H2 produces negative peaks with TCD (µTCD) and helium carrier gas.
Dirty ECD detector can give negative peak after a positive one Clean or replace the ECD detector.
No peaks at all
Possible cause Suggestions
Defective syringe Try a new or proven syringe.
"Blown" septum or massive leaks at the inlet Find and fix leaks.
Problems with carrier gas flow Adjust gas flow. Check the column flow ath the column outlet by immersion to methanol.
Broken column or column installed in the wrong way Replace or reinstall the column.
The detector is not functioning or not connected to the recorder or integrator. Ensure that detector is working properly. E.g.: Is the flame in a FID on? Check connection to the output device.
Selective sensitivity loss
Possible cause Suggestions
Contamination of column and/or liner can lead to loss of sensitivity for active compounds Clean liner. Bake out the column or replace it.
Injector leaks reduce the peak height of the most volatile components of a sample more than less volatile Find and fix any leaks.
Initial column temperature too high for splitless injection which can prevent refocusing of sample. This affects the more volatile components most. Initial column temperature should be below the boiling point of the solvent. Decrease the initial column temperature or use less volatile solvent.
Inlet descrimination. Injector temperature is too low. Later eluting and less volatile compounds have low response. Increase injection temperature.
Split peaks


Possible peaks Suggestions
Fluctuations in column temperature Repair temperature control system
Mixed sample solvent for splitless or on-column injections Use single solvent
When using injection techniques that require "solvent effect" refocusing such as splitless injectiion, the solvent must form a compact, continuous flooded zone in the column. If the solvent does not wet the stationary phase sufficiently as might be the case for methanol used with a nonpolarstationary phase, the solvent flooded zone may be several meters long and not of uniform thickness. This will result in broad and distorted peaks because the solutes will not be refocused into a narrow band near the beginning of the column. Installing a retention gap (5 meters of uncoated but deactivated column) ahead of the crhomatographic column may reduce or eliminate this problem.
Tailing peaks
Possible cause Suggestions
Contaminated or active injector liner, seal or column Clean or replace injector liner. Do not use glass wool in the liner. If necessary, replace the column.
Dead volume due to poorly installed liner or column. Confirm by injecting inert peak (methane). If it tails, column is not properly installed. Reinstall liner and column as necessary.
Ragged column end Score the tubing lightly with a ceramic scoring wafer or sapphire scriber before breaking it. Examine the end using magnifying glass. If the break is not clean and the end square, cut the column again. Point the end down while breaking it and while installing a nut and ferrule to prevent fragments from entering the column. Reinstall the column.
A bad match between the polarities of the stationary phase and the solvent Change the stationaryphase. Usually polar analytes tail on no-polar columns, or dirty columns.
A cold region in the sample flow path Remove any cold zones in the flow path
Debris in the liner or column Clean or replace the liner. Cut 10 cm off the end of the column and reinstall it.
Injection takes too long. Improve injection technique.
Split ration is too low Increase split ratio to at least 20:1
Overloading the inlet Decrease the sample volume or dilute sample
Some types of compounds such as alcoholic amines, primary and secondary amines and carboxylic acids tend to tail. Try a more polar column. Make a derivative of the dsample.
Retention time shifts
Possible cause Suggestions
Change in column temperature Check GC oven temperature
Change in gas flow rate (linear velocity) Inject a detectable unretained sample such as methane to determine the linear gas velocity. Adjust gas pressure or flow to obtain proper values for your analytical method.
Leak in the injector Check the septum first. Change, if necessary. Find the leak and fix it.
Change of solvent Use the same solvent for standards and samples.
Contaminated column Bake out the column. Cut 10 cm off the end of the column. If necessary, replace the column.
Loss of resolution


Possible cause Suggestions
Damage to stationary phase of column Replace the column. This is usually indicated by excessive column bleeding or peak tailing.
Injector problems Check for leaks, inapropriate temperature, split ration, purge time, dirty liner, glass wool in liner.
Large increase in sample concentration
  • Dilute sample
  • Inject less
  • Use higher split ratio
Broad solvent front
Possible cause Suggestions
Bad column installation Reinstall column
Injector leak Find and fix leak
Injection volume too large Decrease sample size or dilute it
Injection temperature too low Increase injection temperature so the entire sample is vaporized "instantly". An injection temperature higher than the temperature limit of the column will not damage the column.
Split ratio is too low Increase split ratio.
Column temperature too low Increase column temperature (ba careful on maximum column temperature limit). Use a lower boiling solvent.
Initial column temperature too high for splitless injection Decrease the initial column temperature. Use a less volatile solvent so the initial column temperature is below the solvent boiling point.
Purge time too long (splitless injection) Use a shorter purge valve close time.
Rapid column performance degradation
Possible cause Suggestions
Broken column Replace column. Avoid damaging the polyimide coating on the column. Avoide temperatures above maximu column temperature limit. Avoid abrasion of the column. Remember, even if the column does not break immediately, when protective coating is damaged the column may possibly break spontaneously later.
Column too hot for too long Replace the column. Stay below limits specified for the column.
Exposure to oxygen, particularly at elevated temperatures Find and fix any lieaks. Be sure carrier gas is sufficiently pure.
Chemical damage due to inorganic acids or bases Keep inorganic acids or bases out of column. Neutralize samples.
Contamination of the column with nonvolatile materials Prevent nonvolatile materials from getting into column. For expample, use a guard column.

Column installation

Detailed information about GC column installation is available here.

toolPreventive maintenance

Injector cleaning
Detector cleaning

GC liners

Inlet LinersFor most chromatographers poor sample reproducibility and mass transfer onto chromatographic column are generally the most critical issues in the method developement. Among others poor reproducibility and mass transfer can be caused by the degradation of the compounds by the glass inlet liner or by means of insufficient vaporization. Therefore selection of proper GC liner is very important and depends on the injection technique. Here you will find important information helping you with selectionof proper GC liner.

Issues surrounding the GC injector ports

New liner desing - FocusLiner™

Siltek deactivation

SGE liner selection guide

Restek TOPAZ™ liners


Syringe Cleaning and Maintenance

Chromatography syringes are the finest quality precision fluid measuring devices available. With proper care and handling, syringes will provide unsurpassed performance in precision fluid measuring year after year. The life of your syringe is directly related to its cleanliness!

Some solvents, such as halogenated hydrocarbons, may attack and deteriorate the highly resistive adhesives (cements) used to affix needles and other terminations to Hamilton syringes, which may result in frozen plungers and plugged needles.


Cleaning Syringe Barrels

To clean Hamilton syringes, it is best to use solvents known to be effective in solvating the sample and preferably are non-alkaline, non-phosphate and non-detergent based. A biodegradable, non-phosphate, organic Cleaning Concentrate is available from Hamilton (ordering number 18311).

Rinse the syringe thoroughly after use with deionized water, acetone, or another solvent compatible with the sample. Allow the syringe to air dry. Avoid prolonged immersion of the syringe while cleaning.

MICROLITER™ Syringes (Series 600, 700, 800 and 900)
  • Rinse the syringe thoroughly with a solvent known to be effective in solvating the sample. Residual dissolved solids may result in frozen plungers and plugged needles.
  • To clean the plunger, remove it from the syringe barrel and gently wipe with a lint-free tissue. Reinsert the plunger into the barrel and pump deionized water, acetone or another solvent compatible with the sample through the needle and syringe. Allow syringe to air dry. When working with dissolved solids, storing the plunger outside of the syringe will reduce the possibility of frozen plunger.
GASTIGHT® Syringes (Series 1000, 1700 and 1800)
  • Rinse the syringe thoroughly with a solvent known to be effective in solvating the sample. Residual dissolve d solids may result in frozen plungers and plugged needles.
  • To clean the plunger, remove it from the syringe barrel and gently wipe with a lint-free tissue. Insert the plunger into the barrel and pump deionized water, acetone or another solvent compatible with the sample through the needle and syringe. Allow syringe to air dry. When working with dissolved solids, storing the plunger outside of the syringe will reduce the possibility of frozen plunger.
Syringe Storage

We recommend to store syringes in the original packaging. This with help to protect the syringe, and allows for easy identification. Remove product description label from the end of the box, and placing it to the outside packaging. This will make re-ordering the same syringe quick and easy.

Handling chiral columns

Lux column use and care

Chiral columnShipping Solvent

n-Hexane/2-propanol (9:1, v/v)

Test Certificate

Each column is individually tested before shipment. A test certificate showing the separation parameters for trans-stilbene oxide is enclosed with each column.

Mobile Phase Compatibility

Lux columns can be used with normal phase (alkane/alcohol), reversed phase (aqueous methanol, aqueous acetonitrile or appropriate buffer/methanol or buffer/acetonitrile mixtures), as well as with pure polar organicsolvents (low molecular weight alcohols, acetonitrile or their mixtures).

Solvent Switching

An appropriate column washing procedure must be applied when changing from one mobile phase to another. The miscibility of the different mobile phase components must be carefully considered for this wash. To safely transfer a column from hexane to methanol (or acetonitrile) or from methanol (or acetonitrile) to hexane, use 100 % 2-propanol as transition solvent at a flow rate of 0.2-0.5 mL/min. Ten column volumes of 2-propanol (i.e. 25 mL for a 250 x 4.6 mm i.d. column or 15 mL for a 150 x 4.6 mm i.d. column) are sufficient for completely removing the old mobile phase. To safely transfer a column from normal phase to reversed phase conditions flush the column with 100 % 2-propanol at 0.2-0.5 mL/min for minimum ten column volumes. In addition, when the buffer salt additive of the RP mobile phase is insoluble in 2-propanol, flush the column briefly with water before switching to a buffered mobile phase. We recommend the use of dedicated Lux columns to reversed phase operation hence avoiding the need of converting columns used in normal phase elution mode to reversed phase or vice versa.

Use of Mobile Phase Modifiers

For basic samples or acidic chiral compounds, it may be necessary to use an appropriate mobile phase modifier in order to achieve chiral resolution and to insure proper peak shapes. Diethylamine, ethanolamine and butyl amine in the concentration range 0.1-0.5 % can be used with basic analytes, while trifluoroacetic or acetic acid (0.1-0.5 %; typically 0.1-0.2 %) with acidic analytes. Mixtures of basic and acidic mobile phase additives are acceptable (e.g. diethyl amine acetate or trifluoroacetate). Lux columns will deliver consistent results when operated with mobile phases containing additives at the concentration levels specified above. However, limited decrease in column efficiency may occur when a column is used in combination with these additives. Therefore, we advise to dedicate columns to mobile phases containing basic additives. Mobile Phase Restrictions Lux chiral stationary phases are prepared by coating silica with various polysaccharide derivatives. Therefore, any solvent dissolving the polysaccharide derivative (such as tetrahydrofurane, acetone, chlorinated hydrocarbons, ethylacetate, dimethylsulfoxide, dimethylformamide, N-methylformamide, etc.) must be avoided even in trace amounts (e.g. even as sample solvent).

Operating Backpressure

The mobile phase flow rate should be set such that the column backpressure stays below 300 bar (4300 psi). This maximum backpressure should not be exceeded for long periods of time.

Operating Temperatures

With standard mobile phases (such as alkane/alcohol) the column can be used in the temperature range 0-50 °C.

Column Storage

Column storage for a longer period of time is recommended in n-hexane/2-propanol (9:1, v/v). Columns used in reversed phase conditions should be first flushed with water (whenever a buffer salt was used as RP mobile phase additive) and then with methanol (or with methanol only when no salt was used). The column can be stored in methanol.

Extending Lifetime and Reconditioning

Phenomenex recommends the use of SecurityGuard™ guard cartridges to extend the lifetime of your column, especially with samples extracted from complex matrixes. Ideally, samples must be completely dissolved in the mobile phase or filtered through a syringe filter of approximately 0.45 μm porosity.

Kinetex columns and solvents

Kinetex columnThere are several critical characteristics that must be taken into consideration when selecting the appropriate organic solvent to use in the mobile phase with Kinetex columns. Viscosity is one of the most important as high viscosity solvents may produce backpressures that are too high for the HPLC system used. Other important solvent characteristics include UV cutoff, cost and polarity index; where a solvent with a high UV cutoff will result in poor sensitivity with UV/Vis detection and use of high cost solvents will result in a poor laboratory that can't afford to buy new columns. Solvents with low polarity indices generally result in faster elution of organic compounds and are commonly used for column cleaning.


is arguably the best organic solvent as it results in the lowest system backpressure in water mixtures and also has a very low UV cutoff for better UV/Vis detection sensitivity. Although acetonitrile production is starting to increase with the turnaround of the economy, thus dropping cost, this still remains a major drawback of acetonitrile usage.


is another popular organic solvent as it is comparable in elution strength to acetonitrile, has a relatively low UV absorbance, and is significantly less expensive than acetonitrile. The major drawback of methanol, especially when used with small particle size HPLC columns, is that its use can result in backpressures that exceed many HPLC system limits.


is less commonly used as it has high UV absorbance, but can be used successfully if analytes absorb at higher UV wavelengths or if other detector types such as MS are used as it has similar elution properties to acetonitrile but is significantly less expensive.


is generally not recommended as it results in very high backpressures in water mixtures.

Iso-, n-propanol

have relatively strong elution strength and are most commonly used in column cleaning at low flow rates as they also results in high backpressures.


has similar elution strength to n-propanol but is less commonly used as it is much more expensive.

GC septa selection

Agilent GCs

Injector type Instrument Dimensions
Split-splitless 7890, 6890, 6850, 5890, 5880A 11 mm
Split-splitless 5880, 5700 9.5 / 10 mm
PTV 7890, 6890, 6850, 5890, 5880A 11 mm
On-column 7890, 6890, 6850, 5890 5 mm


Injector type Instrument Dimensions
Split-splitless Master, GC1000 12 mm
PTV Master, GC1000 12 mm

Perkin-Elmer GCs

Injector type Instrument Dimensions
Split-splitless Auto SYS, Auto SYS XL, 8000, 900, 990, Sigma 11 mm

Shimadzu GCs

Injector type Instrument Dimensions
Split-splitless, PTV 2010, 2014, 17A "plug-septa"

Varian GCs

Injector type Instrument Dimensions
Packed column   9.5 / 10 mm
1079, 1078   10 / 11 mm
1177   9 mm
1075 / 1077   11 mm

Thermo Scientific GCs

Injector type Instrument Dimensions
Split-splitless Trace, 8000, 8000 TOP 17 mm
PTV 8000 17 mm
Split-splitless Trace, GC9001 9.5 mm

Copyright © CHROMSERVIS s.r.o., Jakobiho 327, CZ-109 00 Praha 10 ~ Tel: +420 274 021 211 ~ Znalostní databáze ~ e-shop od MyWebdesign.cz