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          Immuniafinity column materials

          IAC columnsMycotoxins are toxic substances naturally produced by molds (fungi) that may contaminate agricultural commodities by growing on grain or feed. These molds result in a variety of dangerous mycotoxins, such as aflatoxin, fumonisin, deoxynivalenol (DON), ochratoxin, and zearalenone - some of which are known human carcinogens. The U.S. Food & Drug Administration (FDA) has determined acceptable levels of certain mycotoxins for consumption by humans and different species of animals. Furthermore, the European Union (EU) continues to tighten its regulations. This stiffening of EU import standards drives worldwide adoption of mycotoxin tests that are ever more precise, accurate, and sensitive.

          One method of mycotoxin analysis includes sample extraction and clean-up with VICAM immunoaffinity columns, followed by high pressure liquid chromatography (HPLC). In addition to HPLC analysis, VICAM immunoaffinity columns can be used for the quantitative detection of mycotoxin present through the use of a fluorometer. The use of VICAM immunoaffinity columns with the aforementioned instruments can provide a reading of actual mycotoxin content over a range of contamination levels.

          More information about products are available in the product catalogue. List of Official methods using Vicam immunoaffinity columns is available here.

          Vicam Immunoafinity Columns

          Product Name Mycotoxin of interest Detection limit Analytical Method Note
          Myco6in1™ Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Ochratoxine A Fuminonisin B1 Fuminonisin B2 DON Zearalenone T2 Toxine HT-2 Toxine 0.6 ppb 0.3 ppb 0.4 ppb 0.8 ppb 0.6 ppb 1.1 ppb 0.4 ppb 4.2 ppb 0.7 ppb 1.5 ppb 1.9 ppb LC/MS/MS Detection limits are dependent upon the detection system used.
          Aflatest® Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Aflatoxin M1 from 0.1 ppb Fluorometer or HPLC
          Aflatest® WB Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Aflatoxin M1 from 0.03 ppb HPLC 3 ml tube, fast-flow columns, wide range
          Aflatest® WB SR Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Aflatoxin M1 HPLC 3 ml Fast-flow widebore columns are specially designed to maximize aflatoxin G2 recovery and accelerate sample throughput .
          Afla B™ Aflatoxin B1 Aflatoxin B2 from 0.1ppb Fluorometer or HPLC
          Afla M1FL™ Aflatoxin M1 12.5 ppt* Fluorometer
          Afla M1HPLC™ Aflatoxin M1 10 ppt* HPLC
          AflaOchra HPLC™ Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Ochratoxin A from 0.25 ppb HPLC
          AOZ HPLC™ Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Ochratoxin A Zearalenone 0.1 ppb 0.1 ppb 0.1 ppb 0.1 ppb 0.25 ppb 5.0 ppb HPLC
          CitriTest™ Citrinin 10 ppb HPLC
          DONtest™FL+ Deoxynivalenol (DON) 0.5 ppm Fluorometer
          DONtest HPLC™ Deoxynivalenol (DON) 0.1 ppm HPLC
          DONtest™ WB Deoxynivalenol (DON) 40 ppb HPLC 3 ml tube, fast-flow columns, wide range
          FumoniTets™ Fumonisin B1 Fumonisin B2 Fumonisin B3 16 ppb HPLC
          Fumonitest™ WB Fumonisin B1 Fumonisin B2 Fumonisin B3 10 ppm HPLC 3 ml tube, fast-flow columns, wide range
          FumoniTest200™ Fumonisin B1 Fumonisin B2 Fumonisin B3 0.5 ppm Fluorometer
          OchraTest™ Ochratoxin A 1 ppb Fluorometr or HPLC
          Ochratest™ WB Ochratoxin A 0.25 ppb HPLC 3 ml tube, fast-flow columns, wide range
          T-2test™ HPLC T-2 toxin 5.0 ppb HPLC
          T-2/TH-2™ HPLC T-2 toxin TH-2 toxin HPLC
          ZearalaTets™ Zearalenone 0.1 ppm Flourometr or HPLC
          ZearalaTets™ WB Zearalenone 10 ppb HPLC 3 ml tube, fast-flow columns, wide range

          *ppt - parts per trillion