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Purification of vitellogenin from bee haemolymph (APK #508)

Vitellogenins, glycolipophosphoproteins mostly known and well-characterized as precursors of yolk proteins, are involved in reproduction in the majority of oviparous animals. In insects, vitellogenins are typically synthesized in the fat body, from where they are transported via the haemolymph into growing oocytes. The honey bees are no exception: vitellogenin (mw about 200 kDa) is known to play crucial roles in bee reproduction, but its involvement appears to be multifunctional, since vitellogenin influences oxidative stress, nutrition, immunity, and honey bee longevity.