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Testimonials

Novavax CZ

NovavaxWe are currently using ChromShell C18-XB column for analysis of Tweens. The peak shape, peak retention and resolution shows very reproducible results. It is a high-quality and long lasting column.

https://www.novavax.cz/en

Charles University, First Faculty of Medicine

The LION® LN-FAME HT column with dimensions 60 m x 0,25 mm (0,20 µm d.f.) has been used in method development for the fatty acid profiling in plasma lipid classes in our Atherosclerosis Research Laboratory at First Faculty of Medicine, Charles University in Prague, Czechia. This column withstands high temperatures needed to elute cholesterol from the system when analysing cholesteryl ester profiles. Separation of peaks critical for the methyl ester analyses is also possible on this type of column.

RNDr. Marek Vecka, PhD.

EKOCENTRUM OVALAB, s.r.o.

EKOCENTRUM OVALAB, s.r.o. is an independent private laboratory providing analytical and consulting services for producers and importers of food and feed and in the environmental field. The laboratory holds the GMP Certificate issued by SÚKL authority in the scope of physical, physico-chemical and chemical testing of medicines.

We have good experience with the use of ASTRA® and ARION® HPLC columns, in many cases these columns outperform the columns of the world's leading manufacturers. Commonly used methods on these columns are determination of vitamins A, E, C, coenzyme Q10, carotenoid pigments such as lutein, lycopene, zeaxanthin, beta-carotene, amino acids, etc.

All used chromatographic columns are characterized by an excellent price/performance ratio. We recommend them especially for routine laboratories in daily operation.

EKOCENTRUM OVALAB, s.r.o.

EKOCENTRUM OVALAB, s.r.o. is an independent private laboratory providing analytical and consulting services for producers and importers of food and feed and in the environmental field. The laboratory holds the GMP Certificate issued by SÚKL authority in the scope of physical, physico-chemical and chemical testing of medicines.

For our accredited GC methods and analyzes of pharmaceuticals according to GMP standards (fatty acids, sterols, terpenic substances, etc.) we widely use different phases of LION® columns (LN-624, LN-1701, LN-Wax). They provide excellent separation, long life and excellent repeatability.

All used chromatographic columns are characterized by an excellent price/performance ratio. We recommend them especially for routine laboratories in daily operation.

Chempoint

Chempoint, a well-established chemical company headquartered in Slovakia, has been a trusted source of high-quality chemical solutions for over two decades. With a focus on organic chemistry, they are able to synthesize extremely wide variety of compounds, particularly renowned for expertise in organophosphorus compounds and isocyanates.

The chromatography column, LION LN-5 MS, has demonstrated exceptional versatility in analyzing a wide range of compounds. Notably, it has proven resilient even when subjected to highly corrosive and acidic substances, showcasing its robustness in handling hostile chemical environments. Furthermore, it consistently delivers good separation, enhancing the accuracy and reliability of compound analysis

CHEMPOINT – Ing. Miroslav Toma

Nephron Pharmaceuticals Corporation

Nephron Pharmaceutical Corporation is a US leading pharmaceutical manufacturer in the field of inhalation solutions and suspensions. The company has over 26 years of experience manufacturing high-quality, sterile medication for hospitals and medical facilities across the Unites States.

https://www.nephronpharm.com/

The ARION® PLUS C18, 3 µm, 2.1 mm x 100 mm has been used in method development for the Determination of Impurities in both Drug Product and Drug Substance (proprietary information) in our R&D chemistry laboratory at Nephron Pharmaceuticals Corporation. The column does need a guard column fitting (ARION® PLUS 5 mm Cartridge for Guard System, RP, 3.0 µm, ID 2.1 mm) to run this specific method. This column is suitable for use at high temperatures when improved separation is needed. This column also operates in extremely acidic conditions making it ideal for use with this specific test method.

R&D Chemist II – Contract Manufacturing

InventionBio S.A. Laboratory

In our laboratory, MetAmino Kit® was used to determine protein amino acids (such as glycine, serine, arginine, threonine, alanine, proline, valine, lysine, histidine, leucine, phenylalanine, methionine, etc.) in feed samples and lyophilisated intestinal content. Samples after acid hydrolysis were derivatized using MetAmino Kit and finally there were analyzed on LC-MS/MS. The developed method of sample preparation using MetAmino ensures repeatable, reliable analysis results and determination of feed digestibility and amino acid bioavailability.

We recommend using the MetAmino Kit for simple samples derivatization and reliable tool for the identification and quantification of amino acid. It is easy to use and it contributed to the successful results of our research projects.

Anna Filipiak-Szok, Ph.D.

P.J. Šafárik University, Faculty of Medicine

I am satisfied with the MetAmino Kit® because of its complexity. It is possible to quickly obtain necessary results. Each component for the final derivatization is enclosed and the sample derivatization is easy.

We have used it for monitoring of metabolites in STD1 and STD2 in plasma samples from COVID patients with a comparison to a healthy group. The experimental procedure is easily explained. I also appreciate that the column is enclosed in the kit.

RNDr. Soňa Tkáčiková, PhD.

Comenius University, Jessenius Faculty of Medicine

We had the opportunity to use the MetAmino Kit® for liquid chromatography (LC) in combination with mass spectrometry (MS) to determine polar substances in various biological matrices, such as culture media and blood serum or plasma. We can say without hesitation, that we were extremely satisfied with the obtained results. The kit provided us with all the necessary reagents, accessories and clear instructions to perform derivatization and analysis with high accuracy and sensitivity. One aspect that impressed us, was its simplicity of use. The instructions were clear and concise, which allowed us to quickly familiarize ourselves with the process.

The derivatization of substances itself is not so time-consuming and contributes to increasing the sensitivity of the determination of individual compounds. Thus, it reduces the limit of detection or quantification compared to other methods without derivatization. It is therefore possible to determine substances that are present in low concentrations. It is suitable for the analysis of substances that are difficult to quantify, using other methods, such as polyamines. At the same time, it is also possible to determine substances with a low molecular weight that can be degraded in the ion source. From our own experience we can mention glycine, alanine, and serine as problematic, which under normal circumstances can decarboxylate under the influence of high voltage, which we managed to prevent by using the MetAmino kit. Subsequently, we managed to successfully determine them in our samples. It is therefore a suitable complementary method to other methods that are limited in this respect. Another advantage is the short time of the LC-MS analysis itself and the simple evaluation of the obtained data.

Overall, we are more than satisfied with this liquid chromatography and mass spectrometry kit. It provides excellent results, is easy to use and has contributed to the successful execution of our research projects. We recommend it to all laboratories and researchers who need a reliable tool for the identification and quantification of molecules.

Mgr. Radovan Murín, Ph.D. & Mgr. Jakub Šofranko

J.E. Purkyně University

UJEP The MetAmino Kit® was used for the determination of amino acids in soils with a focus on the rhizosphere. The tested soil was contaminated with organic pollutants (diesel, PAH). If we compare the MetAmino Kit with the direct injection method without derivatization, higher and narrower peaks were achieved for the monitored amino acids. The advantage of the kit is the delivery of a complete set for sample preparation including isotopically labeled standards.

Sylvie Kříženecká, PhD.