This Whitepaper … …is written for all chromatographers who deal with the analysis and / or purification of oligonucleotides. The focus is on anion exchange chromatography (AEX) and ion pair reversed phase chromatography (IP-RP). For both modes, the main aspects are the selection of a suitable stationary and mobile phase as well as the optimum temperature. Of course, the special features of the large-scale process are also taken into account.
In this whitepaper, you are guided through the following aspects:
Introduction to Therapeutic Oligonucleotides by Valentina D’Atri, PhD, University of Geneva
Separation Modes in Liquid Chromatography for Therapeutic Oligonucleotides
Anion Exchange Chromatography
Ion-Pairing Reversed Phase Chromatography
Important Considerations for Loadability and Scale-Up