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Sample preparation / Filtration / Filtration media

Sample preparation introduction

Sample preparation

Sample preparation

In sample preparation, there are many techniques used for sample handling, preparation and storage prior the analysis. Main techniques used in this area are:

  • Drying
  • Evaporation
  • Filtration
  • Flash chromatography
  • Homogenization
  • Humidification
  • Immunoaffinity chromatography (IAC for mycotoxines)
  • Preparative chromatography
  • SPE
  • Thermal desorption
  • Ultrassonic extraction

Many samples or laboratory techniques require cooling or heating. That is why folowing instruments are used:

  • Drying ovens
  • Freezers
  • Heating blocks
  • Incubators
  • Refrigerators
  • Sterilizers
  • Thermostatic cabinets
  • Water baths

 

CA Filters

Cellulose Acetate(CA)CA Filters

  • Composition: Mixture of triacetate and diacetate
  • Characteristics:Low static charge and high strhgth
  • Sterilizable: May be repeatedly sterilized without loss of integrity or change in bubble point
  • Clean: 0.1 wt% aqueous extractables
  • Related to MCE membranes: - improved solvend resistance to low molecular weight alcohols - better heat resistance - lower protein binding
    Specifications for Cellulose Acetate (CA)
    ore Size [µm Bubble Point [MPa] Flow rate (Water)[ml/min/cm2] Flow rate (Air) [L/min/cm2] Porosity [%] Thickness [µm]
    0.20 ≥0.25 16 2 66 125
    0.45 ≥0.17 35 4 68 125
    0.80 ≥0.068 160 14 72 125
    3.00 ≥0.034 500 54 78 135

 

 

 

Specifications for CE
Pore Size [µm] Color Surface Bubble Point [MPa] Flow rate (Water)[ml/min/cm2] Flow rate (Air) [L/min/cm2] Porosity [%] Thickness [µm]
0.10 White Plain ≥0.24 2.7 0.67 65 110
0.20 White Plain ≥0.37 17.5 2.4 73 133
0.30 White Plain ≥0.28 30 3.7 75 114
0.45 White Plain ≥0.24 45 5.0 78 145
0.45 White Grid ≥0.16 80 8.0 79 142
0.65 White Plain ≥0.14 120 11.2 79 150
0.80 White Plain ≥0.11 165 15.0 80 150
1.00 White Plain ≥0.096 220 20.4 80 150
3.00 White Plain ≥0.070 300 28.3 81 155
5.00 White Plain ≥0.058 400 40.9 81 160
0.45 Black Grid ≥0.22 50 5.0 78 135
0.80 Black Grid ≥0.10 170 15.0 80 145
0.45 Green Grid ≥0.22 50 5.0 78 135
0.80 Green Grid ≥0.10 170 15.0 80 145

 

 

Ash Content of White Plain MCE Membrane Filters (ppm)
           
Al <0.2 K 6.0 Ni <5.0
Ca 140.0 Li <1.0 Pb <1.0
Cd <0.5 Mg 10.0 Si <20.0
Cr 8.0 Mn <0.5 Sn <5.0
Cu <1.0 Mo <1.0 Ti <1.0
Fe <5.0 Na 10.0 Zn <1.0

MCE Filters

Mixed Cellulose Esters (MCE)MCE Filters

  • Composition: Mixed cellulose esters includes cellulose nitrate, also known as nitrocellulose, and cellulose acetate
  • High porosity: Provide superior flow rates
  • High protein binding: Can be blocked by pretreatment or utilized in applications
  • High purity: Triton-free and non reactive to pyrogens
  • Autoclavable: Withstand autoclaving temperatures up to 130 C without adversely affecting bubble point rate or microbiological recovery
  • Rapid wetting time: <3 seconds to wet a 47 diameter disc with aqueous 1% methylene blue

 

Specifications for MCE
Pore Size [µm] Color Surface Bubble Point [MPa] Flow rate (Water)[ml/min/cm2] Flow rate (Air) [L/min/cm2] Porosity [%] Thickness [µm]
0.10 White Plain ≥0.24 2.7 0.67 65 110
0.20 White Plain ≥0.37 17.5 2.4 73 133
0.30 White Plain ≥0.28 30 3.7 75 114
0.45 White Plain ≥0.24 45 5.0 78 145
0.45 White Grid ≥0.16 80 8.0 79 142
0.65 White Plain ≥0.14 120 11.2 79 150
0.80 White Plain ≥0.11 165 15.0 80 150
1.00 White Plain ≥0.096 220 20.4 80 150
3.00 White Plain ≥0.070 300 28.3 81 155
5.00 White Plain ≥0.058 400 40.9 81 160
0.45 Black Grid ≥0.22 50 5.0 78 135
0.80 Black Grid ≥0.10 170 15.0 80 145
0.45 Green Grid ≥0.22 50 5.0 78 135
0.80 Green Grid ≥0.10 170 15.0 80 145

 

 

Ash Content of White Plain MCE Membrane Filters (ppm)
           
Al <0.2 K 6.0 Ni <5.0
Ca 140.0 Li <1.0 Pb <1.0
Cd <0.5 Mg 10.0 Si <20.0
Cr 8.0 Mn <0.5 Sn <5.0
Cu <1.0 Mo <1.0 Ti <1.0
Fe <5.0 Na 10.0 Zn <1.0

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