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ZearalaTest WB - Immunoafinity HPLC Columns (25/box)

Immunoffinity columns are used for isolation of Zearalenone from samples followed by HPLC. Zearalenone is an estrogenic mycotoxin produced by the fungus Fusarium graminearum. Zearalenone induces feminization in animals at concentrations in feed of about 1 mg/kg.

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Product description

Product description

Immunoffinity columns are used for isolation of Zearalenone from samples followed by HPLC. Zearalenone is an estrogenic mycotoxin produced by the fungus Fusarium graminearum. Zearalenone induces feminization in animals at concentrations in feed of about 1 mg/kg. Higher concentrations (50–100 mg/kg) can interfere with conception, ovulation, implantation, fetal development, and the viability of newborn animals. Increasingly concerned with human ingestion of this mycotoxin, the European Union is considering a full slate of proposed regulations for zearalenone in foods ranging from bread to breakfast cereals.

Using monoclonal antibody-based affinity chromatography, ZearalaTest™WB is a new HPLC-only test for the detection of zearalenone using wide bore immunoaffinity columns. With a total volume of 3 mL, compared to the total of 1 mL in VICAM’s standard columns, ZearalaTest WB allows for a faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.

Benefits

  • Exclusive: Specifically for HPLC use
  • Durable: Long shelf life; requires no refrigeration
  • Versatile: For use with a variety of samples
  • Quick: 20 minutes to isolate toxin*
  • Wide Range: Detects levels as high as 1500 ng
  • Fast Flow: Passes more volume over the column

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Category: Laboratory instruments and accessories / Chromatography / IAC columns

excluding preparation and extraction//

Here’s How Simple Zearalatest WB is to Use:

Extract Sample

  • Grind and weigh sample
  • Add salt and methanol/water mixture
  • Filter

Dilute and Filter

  • Dilute extract
  • Filter

Absorb and Elute

  • Pass filtrate over ZearalaTest WB affinity column
  • Wash column
  • Elute zearalanone and collect in a cuvette

Measure

  • Inject eluate into HPLC
  • Determine zearalanone concentratio
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