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Afla B - Immunoafinity columns for Fluorometer & HPLC (25/box)

Reference: V*G1003

Aflatoxin, a toxin from a naturally occurring mold, is a Group 1 carcinogen, proven to cause cancer in humans. Aflatoxin is made up of the sum of 4 total aflatoxins: B1, B2, G1, and G2; however, in some cases only B1 is regulated. Afla B™ from VICAM is the trusted aflatoxin B test that produces precise numerical results.

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Product description

Product description

Aflatoxin, a toxin from a naturally occurring mold, is a Group 1 carcinogen, proven to cause cancer in humans. Aflatoxin is made up of the sum of 4 total aflatoxins: B1, B2, G1, and G2; however, in some cases only B1 is regulated. Afla B™ from VICAM is the trusted aflatoxin B test that produces precise numerical results. Using monoclonal antibody-based affinity chromatography, Afla B can isolate aflatoxins B1 and B2 from feeds, food, grains, and nuts via fluorometric or HPLC detection. Whereas aflotoxin B1 can be singled out and measured uniquely by HPLC, in fluorometric measurements, total B1 and B2 is reported. Afla B is safe and simple. It can be performed in less than 10 minutes and requires no special skills.

Benefits

  • Convenient: For use with fluorometer or HPLC
  • Durable: Long shelf life; requires no refrigeration
  • Versatile: For use with a variety of samples
  • Quick: Less than 10 minutes to isolate toxin*
  • Wide Range: Detects levels as low as 1 ppb(fluorometer) and 50 ppt (HPLC) and as high as 300 ppb
  • Safe: Requires less toxic materials than other methods

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Category: Laboratory instruments and accessories / Chromatography / IAC columns

excludes preparation and extraction//

Here’s How Simple AFLA B is to Use:

Extract Sample

  • Grind and weigh sample
  • Add salt and methanol/water mixture
  • Filter

Dilute and Filter

  • Dilute extract
  • Filter

Absorb and Elute

  • Pass filtrate over Afla B affinity column
  • Wash column with water
  • Elute aflatoxins with methanol and collect in a cuvette

Measure

  • Add developer and place cuvette into a calibrated fluorometer, or
  • Inject eluate into HPLC
  • Determine aflatoxin concentratio
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